| Title: | Monitoring type 2 diabetes from volatile faecal metabolome in Cushing's syndrome and single Afmid mouse models via a longitudinal study |
| Author(s): | Lourenco C; Kelly D; Cantillon J; Cauchi M; Yon MA; Bentley L; Cox RD; Turner C; |
| Address: | "School of Life, Health & Chemical Sciences, Faculty of Science, Technology, Engineering and Mathematics, The Open University, Walton Hall, Milton Keynes, MK7 6AA, UK. Now at: School of Water, Energy and Environment, Cranfield University, Cranfield, Bedfordshire, MK43 0AL, UK. Department of Mathematics & Statistics, University of Limerick, Limerick, V94 T9PX, Ireland. Mammalian Genetics Unit, MRC Harwell Institute, Harwell Campus, Oxfordshire, OX11 0RD, UK. School of Life, Health & Chemical Sciences, Faculty of Science, Technology, Engineering and Mathematics, The Open University, Walton Hall, Milton Keynes, MK7 6AA, UK. claire.turner@brunel.ac.uk. College of Health & Life Sciences, Brunel University London, Kingston Lane, Uxbridge, Middlesex, UB8 3PH, UK. claire.turner@brunel.ac.uk" |
| DOI: | 10.1038/s41598-019-55339-9 |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "The analysis of volatile organic compounds (VOCs) as a non-invasive method for disease monitoring, such as type 2 diabetes (T2D) has shown potential over the years although not yet set in clinical practice. Longitudinal studies to date are limited and the understanding of the underlying VOC emission over the age is poorly understood. This study investigated longitudinal changes in VOCs present in faecal headspace in two mouse models of T2D - Cushing's syndrome and single Afmid knockout mice. Longitudinal changes in bodyweight, blood glucose levels and plasma insulin concentration were also reported. Faecal headspace analysis was carried out using selected ion flow tube mass spectrometry (SIFT-MS) and thermal desorption coupled to gas chromatography-mass spectrometry (TD-GC-MS). Multivariate data analysis of the VOC profile showed differences mainly in acetic acid and butyric acid able to discriminate the groups Afmid and Cushing's mice. Moreover, multivariate data analysis revealed statistically significant differences in VOCs between Cushing's mice/wild-type (WT) littermates, mainly short-chain fatty acids (SCFAs), ketones, and alcohols, and longitudinal differences mainly attributed to methanol, ethanol and acetone. Afmid mice did not present statistically significant differences in their volatile faecal metabolome when compared to their respective WT littermates. The findings suggested that mice developed a diabetic phenotype and that the altered VOC profile may imply a related change in gut microbiota, particularly in Cushing's mice. Furthermore, this study provided major evidence of age-related changes on the volatile profile of diabetic mice" |
| Keywords: | "Animals Arylformamidase/genetics/*metabolism Blood Glucose/metabolism Cushing Syndrome/diagnosis/*metabolism Diabetes Mellitus, Experimental/diagnosis/metabolism Diabetes Mellitus, Type 2/diagnosis/*metabolism Feces Female Gas Chromatography-Mass Spectrom;" |
| Notes: | "MedlineLourenco, Celia Kelly, Darren Cantillon, Jack Cauchi, Michael Yon, Marianne A Bentley, Liz Cox, Roger D Turner, Claire eng MC_U142661184/MRC_/Medical Research Council/United Kingdom Research Support, Non-U.S. Gov't England 2019/12/13 Sci Rep. 2019 Dec 11; 9(1):18779. doi: 10.1038/s41598-019-55339-9" |
