| Title: | "Identification and expression of candidate chemosensory receptors in the white-spotted flower chafer, Protaetia brevitarsis" |
| Author(s): | Liu H; Zhang X; Liu C; Liu Y; Mei X; Zhang T; |
| Address: | "Agriculture College, Xinyang Agriculture and Forestry University, Xinyang, 464000, P. R. China. Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences/Integrated Pest Management Center of Hebei Province/Key Laboratory of IPM on Crops in Northern Region of North China, Ministry of Agriculture, Baoding, 071000, P. R. China. Cangzhou Technical College, Cangzhou, 061001, P. R. China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, P. R. China. Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences/Integrated Pest Management Center of Hebei Province/Key Laboratory of IPM on Crops in Northern Region of North China, Ministry of Agriculture, Baoding, 071000, P. R. China. cauzht@163.com" |
| DOI: | 10.1038/s41598-019-38896-x |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Accurate detection and recognition of chemical signals play extremely important roles for insects in their survival and reproduction. Chemosensory receptors, including odorant receptors (ORs), ionotropic receptors (IRs) and gustatory receptors (GRs), are involved in detection of volatile signals. In the present study, we aimed to identify candidate chemosensory receptors, and RNA-seq technology was employed to sequence the antennal transcriptome of Protaetia brevitarsis (Coleoptera: Scarabaeidae: Cetoniinae), a native agricultural and horticultural pest in East-Asia. According to the sequence similarity analysis, we identified 72 PbreORs, 11 PbreGRs and eight PbreIRs. Among PbreORs, PbreOR2, PbreOR33 and PbreOR53 were preliminarily classified into pheromone receptors. Further qRT-PCR analysis indicated that 11 PbreORs were specifically expressed in the antennae of male P. brevitarsis, whereas 23 PbreORs were specifically expressed in the female antennae. Our results laid a solid foundation for further functional elucidations of insect chemoreceptors, which could be used as the potential targets of pest management" |
| Keywords: | "Amino Acid Sequence Animals Coleoptera/*metabolism Female Male Receptors, Odorant/genetics/*metabolism Receptors, Pheromone/genetics/*metabolism Sequence Homology, Amino Acid;" |
| Notes: | "MedlineLiu, Hongmin Zhang, Xiaofang Liu, Chunqin Liu, Yongqiang Mei, Xiangdong Zhang, Tao eng Research Support, Non-U.S. Gov't England 2019/03/06 Sci Rep. 2019 Mar 4; 9(1):3339. doi: 10.1038/s41598-019-38896-x" |
