| Title: | An odorant receptor from Anopheles sinensis in China is sensitive to oviposition attractants |
| Author(s): | Liu H; Liu L; Cheng P; Huang X; Gong M; |
| Address: | "Department of Medical Entomology, Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining, 272033, Shandong, People's Republic of China. liuhmm163@163.com. Jining Center for Disease Control and Prevention, Jining, 272033, Shandong, People's Republic of China. Department of Medical Entomology, Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining, 272033, Shandong, People's Republic of China. Department of Medical Entomology, Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining, 272033, Shandong, People's Republic of China. gmq2005@163.com" |
| DOI: | 10.1186/s12936-018-2501-4 |
| ISSN/ISBN: | 1475-2875 (Electronic) 1475-2875 (Linking) |
| Abstract: | "BACKGROUND: Anopheles sinensis is an important vector for the spread of malaria in China. Olfactory-related behaviours, particularly oviposition site seeking, offer opportunities for disrupting the disease-transmission process. RESULTS: This is the first report of the identification and characterization of AsinOrco and AsinOR10 in An. sinensis. AsinOrco and AsinOR10 share 97.49% and 90.37% amino acid sequence identity, respectively, with related sequences in Anopheles gambiae. A functional analysis demonstrated that AsinOrco- and AsinOR10-coexpressing HEK293 cells were highly sensitive to 3-methylindole, but showed no significant differences in response to other test odorants when compared to DMSO. CONCLUSIONS: AsinOrco was characterized as a new member of the Orco ortholog subfamily. AsinOR10, which appears to be a member of the OR2-10 subfamily, is directly involved in identification of oviposition sites. This finding will help to elucidate the molecular mechanisms underlying olfactory signaling in An. sinensis and provide many more molecular targets for eco-friendly pest control" |
| Keywords: | "Amino Acid Sequence Animals Anopheles/genetics/*physiology *Chemotaxis China Female Insect Proteins/chemistry/*genetics/metabolism Oviposition/physiology Pheromones/*physiology Phylogeny Receptors, Odorant/chemistry/*genetics/metabolism Sequence Alignment;" |
| Notes: | "MedlineLiu, Hongmei Liu, Luhong Cheng, Peng Huang, Xiaodan Gong, Maoqing eng 81702034/the National Natural Science Foundation of China/ 81471985/the National Natural Science Foundation of China/ 81672059/the National Natural Science Foundation of China/ 2017M622733/China Postdoctoral Science Foundation funded project/ 2016WS0393/medical and health science and technology development project of Shandong Province/ England 2018/10/07 Malar J. 2018 Oct 5; 17(1):348. doi: 10.1186/s12936-018-2501-4" |
