| Title: | Expression Analysis and Binding Assays in the Chemosensory Protein Gene Family Indicate Multiple Roles in Helicoverpa armigera |
| Author(s): | Li ZQ; Zhang S; Luo JY; Zhu J; Cui JJ; Dong SL; |
| Address: | "State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan, China" |
| DOI: | 10.1007/s10886-015-0574-x |
| ISSN/ISBN: | 1573-1561 (Electronic) 0098-0331 (Linking) |
| Abstract: | "Chemosensory proteins (CSPs) have been proposed to capture and transport hydrophobic chemicals to receptors on sensory neurons. We identified and cloned 24 CSP genes to better understand the physiological function of CSPs in Helicoverpa armigera. Quantitative real-time polymerase chain reaction assays indicate that CSP genes are ubiquitously expressed in adult H. armigera tissues. Broad expression patterns in adult tissues suggest that CSPs are involved in a diverse range of cellular processes, including chemosensation as well as other functions not related to chemosensation. The H. armigera CSPs that were highly transcribed in sensory organs or pheromone glands (HarmCSPs 6, 9, 18, 19), were recombinantly expressed in bacteria to explore their function. Fluorescent competitive binding assays were used to measure the binding affinities of these CSPs against 85 plant volatiles and 4 pheromone components. HarmCSP6 displays high binding affinity for pheromone components, whereas the other three proteins do not show affinities for any of the compounds tested. HarmCSP6 is expressed in numerous cells located in or close to long sensilla trichodea on the antennae of both males and females. These results suggest that HarmCSP6 may be involved in transporting female sex pheromones in H. armigera" |
| Keywords: | "Animals Arthropod Antennae/*metabolism Female Gene Expression Gene Expression Profiling In Situ Hybridization Insect Proteins/genetics/*metabolism Ligands Male Models, Structural Moths/genetics/*metabolism Multigene Family Phylogeny Protein Conformation R;" |
| Notes: | "MedlineLi, Zhao-Qun Zhang, Shuai Luo, Jun-Yu Zhu, Jing Cui, Jin-Jie Dong, Shuang-Lin eng Research Support, Non-U.S. Gov't 2015/04/22 J Chem Ecol. 2015 May; 41(5):473-85. doi: 10.1007/s10886-015-0574-x. Epub 2015 Apr 17" |
