« Previous AbstractFloral Scent Emission from Nectaries in the Adaxial Side of the Innermost and Middle Petals in Chimonanthus praecox    Next AbstractOccurrence and potential human health risks of semi-volatile organic compounds in drinking water from cities along the Chinese coastland of the Yellow Sea »

Front Physiol

Title:		"Identification, Expression Patterns, and Functional Characterization of Chemosensory Proteins in Dendroctonus armandi (Coleoptera: Curculionidae: Scolytinae)"
Author(s):		Li Z; Dai L; Chu H; Fu D; Sun Y; Chen H;
Address:		"College of Forestry, Northwest A&F University, Yangling, China. Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, China. College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China"
Journal Title:		Front Physiol
Year:		2018
Volume:		20180327
Issue:
Page Number:		291 -
DOI:		10.3389/fphys.2018.00291
ISSN/ISBN:		1664-042X (Print) 1664-042X (Electronic) 1664-042X (Linking)
Abstract:		"The Chinese white pine beetle, Dendroctonus armandi Tsai and Li (Coleoptera: Curculionidae: Scolytinae), is a serious pest of coniferous forests in China. Thus, there is considerable interest in developing eco-friendly pest-control methods, with the use of semiochemicals as a distinct possibility. Olfaction is extremely important for fitness of D. armandi because it is the primary mechanism through which the insect locates hosts and mates. Thus, here we characterized nine full-length genes encoding chemosensory proteins (CSPs) from D. armandi. The genes were ubiquitously and multiply expressed across different developmental stages and adult tissues, indicating various roles in developmental metamorphosis, olfaction, and gustation. Ligand-binding assays implied that DarmCSP2 may be the carrier of D. armandi pheromones and various plant host volatiles. These volatiles were identified through RNA interference of DarmCSP2 as: (+)-alpha-pinene, (+)-beta-pinene, (-)-beta-pinene, (+)-camphene, (+)-3-carene, and myrcene. The systematic chemosensory functional analysis of DarmCSP2 in this study clarified the molecular mechanisms underlying D. armandi olfaction and provided a theoretical foundation for eco-friendly pest control"
Keywords:		Dendroctonus armandi Eag RNAi chemosensory proteins fluorescence binding assays olfaction semiochemicals;
Notes:		"PubMed-not-MEDLINELi, Zhumei Dai, Lulu Chu, Honglong Fu, Danyang Sun, Yaya Chen, Hui eng Switzerland 2018/04/11 Front Physiol. 2018 Mar 27; 9:291. doi: 10.3389/fphys.2018.00291. eCollection 2018"