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Pest Manag Sci

Title:		Ligand-binding properties of odorant-binding protein 6 in Athetis lepigone to sex pheromones and maize volatiles
Author(s):		Li LL; Huang JR; Xu JW; Yao WC; Yang HH; Shao L; Zhang HR; Dewer Y; Zhu XY; Zhang YN;
Address:		"Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, College of Life Sciences, Huaibei Normal University, Huaibei 235000, China. Henan Key Laboratory of Crop Pest Control, MOA's Regional Key Lab of Crop IPM in Southern Part of Northern China, Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. Phytotoxicity Research Department, Central Agricultural Pesticide Laboratory, Agricultural Research Center, 7 Nadi El-Seid Street, Dokki 12618, Giza, Egypt"
Journal Title:		Pest Manag Sci
Year:		2022
Volume:		20210904
Issue:		1
Page Number:		52 - 62
DOI:		10.1002/ps.6606
ISSN/ISBN:		1526-4998 (Electronic) 1526-498X (Linking)
Abstract:		"BACKGROUND: Athetis lepigone, a noctuid moth feeding on more than 30 different crops worldwide, has evolved a sophisticated, sensitive, and specific chemosensory system to detect and discriminate exogenous chemicals. Odorant-binding proteins (OBPs) are the most important agent in insect chemosensory systems to be explored as an alternative target for environmentally friendly approaches to pest management. RESULTS: To investigate the olfactory function of A. lepigone OBPs (AlepOBPs), AlepOBP6 was identified and expressed in Escherichia coli. The binding affinity of the recombinant OBP to 20 different ligands was then examined using a competitive binding approach. The results revealed that AlepOBP6 can bind to two sex pheromones and ten maize volatiles, and its conformation stability is pH dependent. We also carried out a structure-function study using different molecular approaches, including structure modeling, molecular docking, and a mutation functional assay to identify amino acid residues (M39, V68, W106, Q107, and Y114) involved in the binding of AlepOBP6 to both sex pheromones and maize volatiles in A. lepigone. CONCLUSION: These results suggest that AlepOBP6 is likely involved in mediating the responses of A. lepigone to sex pheromones and maize volatiles, which may play a pivotal function in mating, feeding, and oviposition behaviors. This study not only provides new insight into the binding mechanism of OBPs to sex pheromones and host volatiles in moths, but also contributes to the discovery of novel target candidates for developing efficient behavior disruptors to control A. lepigone in the future. (c) 2021 Society of Chemical Industry"
Keywords:		"Animals Female Insect Proteins/genetics Ligands Molecular Docking Simulation *Moths Pheromones *Receptors, Odorant/genetics *Sex Attractants Zea mays antennal-binding protein X competitive binding assay maize noctuid odorant-binding protein sex pheromone;"
Notes:		"MedlineLi, Lu-Lu Huang, Jian-Rong Xu, Ji-Wei Yao, Wei-Chen Yang, Hui-Hui Shao, Liang Zhang, Hui-Ru Dewer, Youssef Zhu, Xiu-Yun Zhang, Ya-Nan eng 31970456/National Natural Science Foundation of China/ S202013620012/Anhui College Students' Innovation and Entrepreneurship Training Program/ 202010373096/Anhui College Students' Innovation and Entrepreneurship Training Program/ gxgwfx2019023/Overseas Visiting Program of Outstanding Young Talents Program of Anhui Province, China/ KJ2019A0586/Natural Science Fund of Education Department of Anhui Province, China/ 2008085MC63/Anhui Provincial Natural Science Foundation/ 2020YQ09/Youth Funding of Henan Academy of Agricultural sciences/ England 2021/08/22 Pest Manag Sci. 2022 Jan; 78(1):52-62. doi: 10.1002/ps.6606. Epub 2021 Sep 4"