| Title: | Chemosensory Protein 2 of Male Athetis lepigone Is Involved in the Perception of Sex Pheromones and Maize Volatiles |
| Author(s): | Li JQ; Zhu R; Yao WC; Yu HP; Huang JR; Wang Z; Sun XY; Yuan DH; Sun YY; Emam SS; Dewer Y; Zhu XY; Zhang YN; |
| Address: | "College of Life Sciences, Huaibei Normal University, Huaibei 235000, China. Henan Key Laboratory of Crop Pest Control, MOA's Regional Key Lab of Crop IPM in Southern Part of Northern China, Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China. Phytotoxicity Research Department, Central Agricultural Pesticide Laboratory, Agricultural Research Center, 7 Nadi El-Seid Street, Dokki, Giza 12618, Egypt" |
| ISSN/ISBN: | 1520-5118 (Electronic) 0021-8561 (Linking) |
| Abstract: | "In moths, the interactions between chemosensory proteins (CSPs) and sex pheromones have yet to be comprehensively investigated. Here, we examined the function of AlepCSP2 in male Athetis lepigone based on protein expression, molecular docking, site-directed mutagenesis, fluorescence competitive binding analyses, and RNA interference (RNAi) experiments. We found that AlepCSP2 showed strong binding affinity for two sex pheromones and five maize volatiles and that binding was optimal under neutral conditions. Furthermore, we identified six amino acids as being key residues involved in the interaction between AlepCSP2 and multiple ligands. Further RNAi showed that siCSP2 males displayed consistently lower electroantennography responses to two sex pheromones and three maize volatiles at different dosages tested, and the mating rate also decreased significantly by 37.50%. These findings will contribute to characterizing the binding mechanisms of moth CSPs to sex pheromones and host volatiles and also identify unique targets for developing novel pest behavior disruptors" |
| Keywords: | Male Animals *Sex Attractants/metabolism Zea mays/genetics/metabolism Molecular Docking Simulation *Moths/metabolism Insect Proteins/metabolism Perception Pheromones/metabolism Athetis lepigone RNAi chemosensory protein maize volatile sex pheromone site-d; |
| Notes: | "MedlineLi, Jian-Qiao Zhu, Rui Yao, Wei-Chen Yu, Hui-Ping Huang, Jian-Rong Wang, Zhen Sun, Xin-Yue Yuan, Di-Hua Sun, Yuan-Yuan Emam, Sekina S Dewer, Youssef Zhu, Xiu-Yun Zhang, Ya-Nan eng 2023/04/18 J Agric Food Chem. 2023 Apr 26; 71(16):6277-6287. doi: 10.1021/acs.jafc.3c00565. Epub 2023 Apr 17" |
