| Title: | "Effect of psoralen on the expression of PPARgamma, osteocalcin, and trabecular bone area in rabbits with steroid-induced avascular necrosis of the femoral head" |
| Author(s): | Li H; Meng D; Zhang X; Yuan D; |
| Address: | "Orthopedics Department, The First Affiliated Hospital of Henan University of Chinese Medicine, 19 Renmin Road, Zhengzhou, 450000, Henan, China. huiyingli_hntcm@126.com. Orthopedics Department, The First Affiliated Hospital of Henan University of Chinese Medicine, 19 Renmin Road, Zhengzhou, 450000, Henan, China" |
| DOI: | 10.1186/s13018-018-1054-0 |
| ISSN/ISBN: | 1749-799X (Electronic) 1749-799X (Linking) |
| Abstract: | "OBJECTIVE: Psoralen is a natural plant toxin which has the function of protecting fungi, insects, and herbivores. In this study, we aim to investigate the effect and mechanism of psoralen on steroid-induced avascular necrosis of femoral head (SANFH). METHODS: Thirty rabbits were randomly divided into blank group (n = 10), model group (n = 10), and experimental group (n = 10). Rabbits in blank and model groups were treated with normal saline, and rabbits in experimental group were treated with psoralen. Total RNA of bone marrow was extracted by trizol, and the mRNA expression of PPARgamma and osteocalcin were detected by q-PCR. Then, the mRNA expression of PPARgamma and osteocalcin in the three groups were compared. Western blot was used to detect the PPARgamma protein expression in the bone of rabbits. ELISA was used to measure the osteocalcin protein. RESULTS: The mRNA expression of PPARgamma in model group significantly increased compared with blank group. The mRNA expression of osteocalcin in model group decreased compared with blank group. However, the mRNA and protein expressions of PPARgamma in experimental group were significantly decreased compared with the model group. The protein expressions of osteocalcin increased compared with the model group. There was no significant difference of trabecular bone area (TBA) between experimental and blank groups (P > 0.05). TBA in model group was lower than the experimental group (P < 0.05). There was no significant difference of TBA between experimental and blank groups (P > 0.05). CONCLUSION: This research confirms that psoralen plays a positive role in the rehabilitation of SANFH" |
| Keywords: | Animals Cancellous Bone/drug effects/*metabolism Femur Head Necrosis/chemically induced/drug therapy/*metabolism Ficusin/*pharmacology/therapeutic use Gene Expression Osteocalcin/*biosynthesis/genetics PPAR gamma/*biosynthesis/genetics Rabbits Random Allo; |
| Notes: | "MedlineLi, Huiying Meng, Dongfang Zhang, Xiaorui Yuan, Dong eng 15A360004/Key research projects of higher education in Henan province/ England 2019/01/10 J Orthop Surg Res. 2019 Jan 8; 14(1):11. doi: 10.1186/s13018-018-1054-0" |
