| Title: | "Evidence for the Involvement of the Chemosensory Protein AgosCSP5 in Resistance to Insecticides in the Cotton Aphid, Aphis gossypii" |
| Author(s): | Li F; Venthur H; Wang S; Homem RA; Zhou JJ; |
| Address: | "Department of Plant Protection, Hainan University, Haikou 570228, China. Laboratorio de Quimica Ecologica, Departamento de Ciencias Quimicas y Recursos Naturales, Centro de Investigacion Biotecnologica, Aplicada al Medio Ambiente (CIBAMA), Universidad de La Frontera, Temuco 54-D, Chile. College of Plant Sciences, Jilin University, Changchun 130062, China. Department of Biointeractions and Crop Protection, Rothamsted Research, Harpenden AL5 2JQ, UK. Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Gansu Agricultural University, No. 1 Yingmen Village, Anning District, Lanzhou 730070, China. State Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang 550025, China" |
| ISSN/ISBN: | 2075-4450 (Print) 2075-4450 (Electronic) 2075-4450 (Linking) |
| Abstract: | "It has been speculated that insect chemosensory proteins (CSPs) may have additional roles beyond olfaction. In this study, the phylogenetic and genomic analyses of the CSPs of the cotton aphid, Aphis gossypii, revealed the presence of gene gain-and-loss among different aphid field populations. Differential expressions of eight CSP genes were demonstrated after treatments with insecticides of different modes of action. The expression of AgosCSP5 was significantly upregulated by the insecticide treatments in a dose-dependent manner. The Drosophila flies overexpressing AgosCSP5 were significantly less susceptible to the insecticides, omethoate, imidacloprid and cypermethrin but not to deltamethrin and tau-fluvalinate, compared with control flies. The transgenic Drosophila flies exhibited an LC(50) resistance ratio of 2.6 to omethoate, compared with control flies. Likewise, the mortality of the transgenic flies to imidacloprid and cypermethrin was significantly lower than that of the control flies (p < 0.01). Homology modelling, molecular docking and dynamic simulation supported the interactions and revealed a higher stability of AgosCSP5/insecticide complexes than AgosCSP5/semiochemical complexes. Our study demonstrates for first time the in vivo evidence for the involvement of CSP genes in insecticide resistance of crop insect pests and provides new insights of the newly discovered CSP-mediated insect resistance mechanism to insecticides" |
| Keywords: | chemosensory protein insect pest management pesticide resistance; |
| Notes: | "PubMed-not-MEDLINELi, Fen Venthur, Herbert Wang, Shang Homem, Rafael A Zhou, Jing-Jiang eng 31901894/National Natural Science Foundation of China/ 320RC489/Hainan Provincial Natural Science Foundation of China/ D20023/Program of Introducing Talents to Chinese Universities/ GCRF-IAA/the Global Challenge Research Fund/ Switzerland 2021/05/01 Insects. 2021 Apr 9; 12(4):335. doi: 10.3390/insects12040335" |
