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J Biosci Bioeng

Title:		Development of novel on-line capillary gas chromatography-based analysis method for volatile organic compounds produced by aerobic fermentation
Author(s):		Lee HW; Park JH; Lee HS; Kim CS; Lee JG; Kim WK; Ryu KH; Ahn JO; Lee EG; Kim SW; Jeon JM; Yang YH; Choi ES; Lee HW;
Address:		"Biotechnology Process Engineering Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), 30 Yeongudanji-ro, Cheongwon-gu, Cheongju, Chungcheongbuk-do 28116, Republic of Korea; Department of Microbial Engineering, College of Engineering, Konkuk University, 120 Neungdong-ro, Gwanngjin-gu, Seoul 05029, Republic of Korea. Bio-Evaluation Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), Yeongudanji-ro, Cheongwon-gu, Cheongju, Chungcheongbuk-do 28116, Republic of Korea. Biotechnology Process Engineering Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), 30 Yeongudanji-ro, Cheongwon-gu, Cheongju, Chungcheongbuk-do 28116, Republic of Korea; Department of Bioprocess Engineering, University of Science and Technology (UST) of Korea, 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea. Biotechnology Process Engineering Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), 30 Yeongudanji-ro, Cheongwon-gu, Cheongju, Chungcheongbuk-do 28116, Republic of Korea. Division of Applied Life Science (BK21 Plus), PMBBRC, Institute of Agricultural and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea. Department of Microbial Engineering, College of Engineering, Konkuk University, 120 Neungdong-ro, Gwanngjin-gu, Seoul 05029, Republic of Korea. Biotechnology Process Engineering Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), 30 Yeongudanji-ro, Cheongwon-gu, Cheongju, Chungcheongbuk-do 28116, Republic of Korea; Department of Bioprocess Engineering, University of Science and Technology (UST) of Korea, 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea. Electronic address: hwlee@kribb.re.kr"
Journal Title:		J Biosci Bioeng
Year:		2019
Volume:		20180730
Issue:		1
Page Number:		121 - 127
DOI:		10.1016/j.jbiosc.2018.07.007
ISSN/ISBN:		1347-4421 (Electronic) 1347-4421 (Linking)
Abstract:		"Many volatile compounds, such as isoprene, a precursor used in the synthesis of natural rubber, have been produced through fermentation using genetically engineered microorganisms. Despite this biotechnological success, measuring the concentrations of volatile compounds during fermentation is difficult because of their high volatility. In current systems, off-line analytical methods usually lead to product loss, whereas on-line methods raise the production cost due to the requirement of complex devices. Here, we developed a novel on-line gas chromatography (GC)-based system for analyzing the concentration of isoprene with the aim to minimize the cost and requirement for devices as compared to current strategies. In this system, a programmable logic controller is used to combine conventional GC with a syringe pump module (SPM) directly connected to the exhaust pipe of the fermentor, and isoprene-containing samples are continuously pumped from the SPM into the GC using an air cylinder recycle stream. We showed that this novel system enables isoprene analysis during fermentation with convenient equipment and without the requirement of an expensive desorption tube. Furthermore, this system may be extended to the detection of other volatile organic compounds in fermentation or chemical processes"
Keywords:		"Aerobiosis Bioreactors Butadienes/chemistry/metabolism *Capillary Electrochromatography/instrumentation/methods Chromatography, Gas/instrumentation/methods Fermentation/*physiology Gas Chromatography-Mass Spectrometry/instrumentation/methods Hemiterpenes/;"
Notes:		"MedlineLee, Hyeok-Won Park, Jung-Ho Lee, Hee-Suk Kim, Chun-Suk Lee, Jin-Gyeom Kim, Won-Kyo Ryu, Kyoung-Hwa Ahn, Jung-Oh Lee, Eun-Gyo Kim, Seon-Won Jeon, Jong-Min Yang, Yung-Hun Choi, Eui-Sung Lee, Hong-Weon eng Japan 2018/08/04 J Biosci Bioeng. 2019 Jan; 127(1):121-127. doi: 10.1016/j.jbiosc.2018.07.007. Epub 2018 Jul 30"