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Environ Pollut

Title:		Comparative study on gene expression profile in rat lung after repeated exposure to diesel and biodiesel exhausts upstream and downstream of a particle filter
Author(s):		Lecureur V; Monteil C; Jaguin M; Cazier F; Preterre D; Corbiere C; Gosset P; Douki T; Sichel F; Fardel O;
Address:		"Univ Rennes, Inserm, EHESP, Irset (Institut de Recherche en Sante, Environnement et Travail), UMR_S 1085, 35000, Rennes, France. Electronic address: valerie.lecureur@univ-rennes1.fr. Normandie Univ, UNIROUEN, UNICAEN, ABTE, 14000, Caen et 76000 Rouen, France. Univ Rennes, Inserm, EHESP, Irset (Institut de Recherche en Sante, Environnement et Travail), UMR_S 1085, 35000, Rennes, France. Common Center of Measurements (CCM), Univ. Littoral Cote d'Opale, 59140, Dunkerque, France. CERTAM, 1 rue Joseph Fourier, 76800, Saint-Etienne du Rouvray, France. Unite de Chimie Environnementale et Interactions sur le Vivant, EA4492, Universite du Littoral Cote d'Opale, Dunkerque, France. Univ. Grenoble Alpes, CEA, CNRS, INAC, SyMMES/CIBEST, F-38000, Grenoble, France. Normandie Univ, UNIROUEN, UNICAEN, ABTE, 14000, Caen et 76000 Rouen, France; Centre Francois Baclesse, 14000, Caen, France. Univ Rennes, CHU Rennes, Inserm, EHESP, Irset (Institut de Recherche en Sante, Environnement et Travail), UMR_S 1085, 35000, Rennes, France"
Journal Title:		Environ Pollut
Year:		2020
Volume:		20200726
Issue:		Pt 2
Page Number:		115264 -
DOI:		10.1016/j.envpol.2020.115264
ISSN/ISBN:		1873-6424 (Electronic) 0269-7491 (Linking)
Abstract:		"Biodiesel is considered as a valuable and less toxic alternative to diesel. However, cellular and molecular effects of repeated exposure to biodiesel emissions from a recent engine equipped with a diesel particle filter (DPF) remain to be characterized. To gain insights about this point, the lung transcriptional signatures were analyzed for rats (n = 6 per group) exposed to filtered air, 30% rapeseed biodiesel (B30) blend or reference diesel (RF0), upstream and downstream a DPF, for 3 weeks (3 h/day, 5 days/week). Genomic analysis revealed a modest regulation of gene expression level (lower than a 2-fold) by both fuels and a higher number of genes regulated downstream the DPF than upstream, in response to either RF0 or to B30 exhaust emissions. The presence of DPF was found to notably impact the lung gene signature of rats exposed to B30. The number of genes regulated in common by both fuels was low, which is likely due to differences in concentrations of regulated pollutants in exhausts, notably for compound organic volatiles, polycyclic aromatic hydrocarbons, NO or NOx. Nevertheless, we have identified some pathways that were activated for both exhaust emissions, such as integrin-, IGF-1- and Rac-signaling pathways, likely reflecting the effects of gas phase products. By contrast, some canonical pathways relative to 'oxidative phosphorylation' and 'mitochondrial dysfunction' appear as specific to B30 exhaust emission; the repression of transcripts of mitochondrial respiratory chain in lung of rats exposed to B30 downstream of DPF supports the perturbation of mitochondria function. This study done with a recent diesel engine (compliant with the European IV emission standard) and commercially-available fuels reveals that the diesel blend composition and the presence of an after treatment system may modify lung gene signature of rats repeatedly exposed to exhaust emissions, however in a rather modest manner"
Keywords:		Air Pollutants/*analysis Animals Biofuels/*analysis Gasoline/analysis Particulate Matter/analysis Rats Transcriptome Vehicle Emissions/analysis Biodiesel Diesel exhaust Lung Particle filter Transcriptome analysis;
Notes:		"MedlineLecureur, Valerie Monteil, Christelle Jaguin, Marie Cazier, Fabrice Preterre, David Corbiere, Cecile Gosset, Pierre Douki, Thierry Sichel, Francois Fardel, Olivier eng England 2020/08/11 Environ Pollut. 2020 Nov; 266(Pt 2):115264. doi: 10.1016/j.envpol.2020.115264. Epub 2020 Jul 26"