| Title: | Investigation of VOCs associated with different characteristics of breast cancer cells |
| Author(s): | Lavra L; Catini A; Ulivieri A; Capuano R; Baghernajad Salehi L; Sciacchitano S; Bartolazzi A; Nardis S; Paolesse R; Martinelli E; Di Natale C; |
| Address: | "Labotatory of Biomedical Research 'Fondazione Niccolo Cusano per la Ricerca Medico-Scientifica', Niccolo Cusano University, Rome, Italy. Department of Electronic Engineering, University of Rome Tor Vergata, Via del Politecnico 1, 00133 Rome, Italy. Department of Clinical and Molecular Medicine, University of Rome 'Sapienza', Rome, Italy. Department of Pathology, Universitary Hospital Sant'Andrea, Rome, Italy. Department of Oncology-Pathology, Cancer Center Karolinska, Karolinska Hospital, Stockholm, Sweden. Department of Chemical science and technology, University of Rome Tor Vergata, Via di Tor Vergata, 00133 Rome, Italy" |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "The efficacy of breath volatile organic compounds (VOCs) analysis for the screening of patients bearing breast cancer lesions has been demonstrated by using gas chromatography and artificial olfactory systems. On the other hand, in-vitro studies suggest that VOCs detection could also give important indications regarding molecular and tumorigenic characteristics of tumor cells. Aim of this study was to analyze VOCs in the headspace of breast cancer cell lines in order to ascertain the potentiality of VOCs signatures in giving information about these cells and set-up a new sensor system able to detect breast tumor-associated VOCs. We identified by Gas Chromatography-Mass Spectrometry analysis a VOCs signature that discriminates breast cancer cells for: i) transformed condition; ii) cell doubling time (CDT); iii) Estrogen and Progesterone Receptors (ER, PgR) expression, and HER2 overexpression. Moreover, the signals obtained from a temperature modulated metal oxide semiconductor gas sensor can be classified in order to recognize VOCs signatures associated with breast cancer cells, CDT and ER expression. Our results demonstrate that VOCs analysis could give clinically relevant information about proliferative and molecular features of breast cancer cells and pose the basis for the optimization of a low-cost diagnostic device to be used for tumors characterization" |
| Keywords: | "Biomarkers/analysis Breast Neoplasms/*chemistry/*diagnosis Cell Line, Tumor *Electronic Nose Gas Chromatography-Mass Spectrometry/*methods Humans MCF-7 Cells Odorants/analysis Reproducibility of Results Sensitivity and Specificity Volatile Organic Compoun;" |
| Notes: | "MedlineLavra, Luca Catini, Alexandro Ulivieri, Alessandra Capuano, Rosamaria Baghernajad Salehi, Leila Sciacchitano, Salvatore Bartolazzi, Armando Nardis, Sara Paolesse, Roberto Martinelli, Eugenio Di Natale, Corrado eng Evaluation Study Research Support, Non-U.S. Gov't England 2015/08/26 Sci Rep. 2015 Aug 25; 5:13246. doi: 10.1038/srep13246" |
