| Title: | Chemo-profiling of bioactive metabolites from Chaetomium globosum for biocontrol of Sclerotinia rot and plant growth promotion |
| Author(s): | Kumar R; Kundu A; Dutta A; Saha S; Das A; Bhowmik A; |
| Address: | "Division of Agricultural Chemicals, ICAR-Indian Agricultural Research Institute, New Delhi, India. Division of Agricultural Chemicals, ICAR-Indian Agricultural Research Institute, New Delhi, India. Electronic address: chem.aditi@gmail.com. Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, India. Division of Design of Experiment, ICAR-Indian Agricultural Statistics Research Institute, New Delhi, India" |
| DOI: | 10.1016/j.funbio.2020.07.009 |
| Abstract: | "Chaetomium globosum Kunze ex. Fries has been known to produce diverse bioactive metabolites, attracting researchers to exploit the biocontrol agent for plant disease management. However, distinct research gaps are visible regarding detail characterization of bioactive metabolites. Thus the current study has been planned to characterize volatile and nonvolatile compounds of most potential strain of C. globosum 5157. GC-MS analysis of hexane fraction revealed twenty-six volatile organic compounds, representing 65.5% of total components in which 3-octanone (21.4%) was found to be most abundant. UPLC-QTOF-MS/MS analysis of ethyl acetate and methanolic fractions resulted tentative characterization of fifteen and eleven metabolites, respectively. Among these, nine metabolites were isolated, purified and characterized using (1)H NMR and High resolution mass spectrometric analysis to delineate mass fragmentation pattern for the first time. Antifungal potential of hexane fraction exhibited high inhibitory action against Sclerotium rolfsii (139.2 mug mL(-1)) whereas ethyl acetate fraction was highly effective against Sclerotinia sclerotiorum (112.1 mug mL(-1)). Comparative assessment of C. globosum 5157 vis a vis Trichoderma harzianum A28 revealed promising effect of C. globosum 5157 with respect to antifungal properties and plant growth promotion of Brassica seedlings" |
| Keywords: | Ascomycota Basidiomycota *Chaetomium Hypocreales Tandem Mass Spectrometry Antifungal activity Biocontrol Chaetomium sp.Gc-ms UHPLC-QToF-MS Volatile organic compounds; |
| Notes: | "MedlineKumar, Rakesh Kundu, Aditi Dutta, Anirban Saha, Supradip Das, Amrita Bhowmik, Arpan eng Research Support, Non-U.S. Gov't Netherlands 2021/02/25 Fungal Biol. 2021 Mar; 125(3):167-176. doi: 10.1016/j.funbio.2020.07.009. Epub 2020 Aug 8" |
