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Anal Chem

Title:		Measurement of 18 perfluorinated organic acids and amides in human serum using on-line solid-phase extraction
Author(s):		Kuklenyik Z; Needham LL; Calafat AM;
Address:		"Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA. ZKuklenyik@cdc.gov"
Journal Title:		Anal Chem
Year:		2005
Volume:		77
Issue:		18
Page Number:		6085 - 6091
DOI:		10.1021/ac050671l
ISSN/ISBN:		0003-2700 (Print) 0003-2700 (Linking)
Abstract:		"We have developed an on-line solid-phase extraction (SPE) method coupled to high-performance liquid chromatography (HPLC)-tandem mass spectrometry (MS/MS) for measuring trace levels of 18 perfluorinated chemicals (3 perfluorosulfonates, 8 perfluorocarboxylates, 7 perfluorosulfonamides) in serum. Without protein precipitation, only dilution with 0.1 M formic acid, one aliquot of 100 microL of serum was injected into a commercial column switching system that allowed for concurrent SPE and HPLC-MS/MS acquisition. First, the analytes were concentrated on a C18 SPE column. Then, this column was placed automatically in front of a C8 analytic HPLC column for chromatographic separation of the analytes. Detection and quantification were done using negative-ion TurboIonSpray ionization, a variant of electrospray ionization, MS/MS. Excellent recovery was achieved for all analytes including the volatile sulfonamide derivatives that could not be determined before using traditional off-line SPE methods. The high throughput and low limits of detection (0.05-0.8 ng/mL) using a small sample volume (100 microL of serum) and isotope dilution quantification make this method suitable for large-scale epidemiologic studies"
Keywords:		"Acids/chemistry Amides/*blood/chemistry Chromatography, High Pressure Liquid Fluorine Compounds/*blood/chemistry Humans Online Systems/*instrumentation Organic Chemicals/*blood/chemistry Solid Phase Extraction/*instrumentation/*methods Tandem Mass Spectro;"
Notes:		"MedlineKuklenyik, Zsuzsanna Needham, Larry L Calafat, Antonia M eng 2005/09/15 Anal Chem. 2005 Sep 15; 77(18):6085-91. doi: 10.1021/ac050671l"