| Title: | cDNA cloning of an adult male putative lipocalin specific to tergal gland aphrodisiac secretion in an insect (Leucophaea maderae) |
| Author(s): | Korchi A; Brossut R; Bouhin H; Delachambre J; |
| Address: | "Developpement-Communication chimique, Unite Mixte de Recherche 5548 du Centre National de la Recherche Scientifique, Universite de Bourgogne, Dijon, France" |
| DOI: | 10.1016/s0014-5793(99)00414-7 |
| ISSN/ISBN: | 0014-5793 (Print) 0014-5793 (Linking) |
| Abstract: | Lma-P22 is a cuticular surface protein specific to the tergal gland secretion of Leucophaea maderae adult males which is ingested by females just before copulation. The complete Lma-P22 cDNA sequence was determined by RT-PCR using primers based on Edman degradation fragments. The recombinant protein expressed in Escherichia coli was recognized by an anti-Lma-P22 antibody. Northern blot analysis indicates that the corresponding mRNA is transcribed only in the epidermis of male tergites. Sequence analysis indicated that Lma-P22 deduced protein belongs to the lipocalin family. Lipocalins are extracellular proteins which carry hydrophobic compounds and some of them can bind sexual pheromone in vertebrates. Lma-P22 is the first example of a lipocalin-like protein involved in insect sexual behavior |
| Keywords: | "Amino Acid Sequence Animals Base Sequence Cloning, Molecular Cockroaches/*genetics DNA, Complementary Escherichia coli Gene Expression Humans Insect Proteins/*genetics Male Molecular Sequence Data RNA, Messenger Recombinant Fusion Proteins/genetics Sequen;" |
| Notes: | "MedlineKorchi, A Brossut, R Bouhin, H Delachambre, J eng Research Support, Non-U.S. Gov't England 1999/05/25 FEBS Lett. 1999 Apr 23; 449(2-3):125-8. doi: 10.1016/s0014-5793(99)00414-7" |
