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Eukaryot Cell

Title:		Functional characterization of MAT1-1-specific mating-type genes in the homothallic ascomycete Sordaria macrospora provides new insights into essential and nonessential sexual regulators
Author(s):		Klix V; Nowrousian M; Ringelberg C; Loros JJ; Dunlap JC; Poggeler S;
Address:		"Institute of Microbiology and Genetics, Department of Genetics of Eukaryotic Microorganisms, Georg-August University, Grisebachstrasse 8, Gottingen, Germany"
Journal Title:		Eukaryot Cell
Year:		2010
Volume:		20100430
Issue:		6
Page Number:		894 - 905
DOI:		10.1128/EC.00019-10
ISSN/ISBN:		1535-9786 (Electronic) 1535-9778 (Print) 1535-9786 (Linking)
Abstract:		"Mating-type genes in fungi encode regulators of mating and sexual development. Heterothallic ascomycete species require different sets of mating-type genes to control nonself-recognition and mating of compatible partners of different mating types. Homothallic (self-fertile) species also carry mating-type genes in their genome that are essential for sexual development. To analyze the molecular basis of homothallism and the role of mating-type genes during fruiting-body development, we deleted each of the three genes, SmtA-1 (MAT1-1-1), SmtA-2 (MAT1-1-2), and SmtA-3 (MAT1-1-3), contained in the MAT1-1 part of the mating-type locus of the homothallic ascomycete species Sordaria macrospora. Phenotypic analysis of deletion mutants revealed that the PPF domain protein-encoding gene SmtA-2 is essential for sexual reproduction, whereas the alpha domain protein-encoding genes SmtA-1 and SmtA-3 play no role in fruiting-body development. By means of cross-species microarray analysis using Neurospora crassa oligonucleotide microarrays hybridized with S. macrospora targets and quantitative real-time PCR, we identified genes expressed under the control of SmtA-1 and SmtA-2. Both genes are involved in the regulation of gene expression, including that of pheromone genes"
Keywords:		"Fungal Proteins/*genetics/metabolism Gene Deletion *Genes, Mating Type, Fungal Mutation Phenotype Polymerase Chain Reaction Sordariales/*genetics/growth & development;"
Notes:		"MedlineKlix, V Nowrousian, M Ringelberg, C Loros, J J Dunlap, J C Poggeler, S eng P01 GM068087/GM/NIGMS NIH HHS/ R01 GM034985/GM/NIGMS NIH HHS/ R01 GM034985-26/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't 2010/05/04 Eukaryot Cell. 2010 Jun; 9(6):894-905. doi: 10.1128/EC.00019-10. Epub 2010 Apr 30"