| Title: | A high-throughput platform for interpretation of metabolite profile data from pepper (Capsicum) fruits of 13 phenotypes associated with different fruit maturity states |
| Author(s): | Kim TJ; Hyeon H; Park NI; Yi TG; Lim SH; Park SY; Ha SH; Kim JK; |
| Address: | "Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National Univ., Incheon 22012, Republic of Korea. Department of Plant Science, Gangneung-Wonju National University, 7 Jukheon-gil, Gangneung 25457, Republic of Korea. National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do 55365, Republic of Korea. Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea. Electronic address: sunhwa@khu.ac.kr. Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National Univ., Incheon 22012, Republic of Korea. Electronic address: kjkpj@inu.ac.kr" |
| DOI: | 10.1016/j.foodchem.2020.127286 |
| ISSN/ISBN: | 1873-7072 (Electronic) 0308-8146 (Linking) |
| Abstract: | "Nowadays, novel tools have been developed for efficient analysis and visualization of large-scale metabolite profile data associated with metabolic pathways. A high-throughput platform using PathVisio 3 combined with multivariate analysis is proposed for the first time. Additionally, this is the first analysis of the relationships among terpenoids monoterpene, sesquiterpene, triterpene, and tetraterpene during pepper fruit ripening, and their changes. This platform was successfully applied to interpret large-scale data related to 131 metabolites from mature and immature fruits of 13 pepper phenotypes. The carotenoid-derived volatiles, such as dihydroactinidiolide and beta-ionone were closely correlated with carotenoids, indicating that the synthesis and degradation of carotenoids occurred in pepper fruit mature stage. Using PathVisio 3, the metabolic changes in pathway could be presented quickly, revealing the accumulation of stress-related metabolites, such as proline, capsaicin, and phenylalanine, in the mature stage. This approach could provide useful information about comprehensive biochemical regulation of fruit ripening" |
| Keywords: | "Capsaicin/analysis/metabolism Capsicum/chemistry/growth & development/*metabolism Carotenoids/analysis/metabolism Chromatography, High Pressure Liquid Cluster Analysis Fruit/chemistry/*growth & development/*metabolism Gas Chromatography-Mass Spectrometry;" |
| Notes: | "MedlineKim, Tae Jin Hyeon, Hyejin Park, Nam Il Yi, Tae Gyu Lim, Sun-Hyung Park, Soo-Yun Ha, Sun-Hwa Kim, Jae Kwang eng England 2020/06/21 Food Chem. 2020 Nov 30; 331:127286. doi: 10.1016/j.foodchem.2020.127286. Epub 2020 Jun 11" |
