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Food Chem

Title:		High throughput quantitative volatile profiling of melons with silicone rod extraction - thermal desorption - GC-MS for plant breeding line selection
Author(s):		Kende A; Lim PP; Lai F; Jessop M; Swindale L; Oliver M; Hurr B; Rickett D; Baxter C;
Address:		"Syngenta UK, Jealott's Hill Research Centre, Bracknell, United Kingdom. Electronic address: aniko.kende@syngenta.com. Syngenta UK, Jealott's Hill Research Centre, Bracknell, United Kingdom. Syngenta France, SAS 12, chemin de l'Hobit BP 27, F-31790 Saint-Sauveur, France. Syngenta Seeds, 21435 Road 98, Woodland, CA 95695, USA"
Journal Title:		Food Chem
Year:		2019
Volume:		20180717
Issue:
Page Number:		368 - 374
DOI:		10.1016/j.foodchem.2018.07.101
ISSN/ISBN:		1873-7072 (Electronic) 0308-8146 (Linking)
Abstract:		"Volatile compounds determine the aroma of fruits, giving their unique flavor characteristics. The aim of many plant breeding projects is to improve the consumers' flavor experience when eating fresh produce. Large scale breeding trials produce thousands of samples which need volatile profiling amongst other phenotypes. Despite this interest, current methods have limitations: sampling unsuitable for field conditions, high cost and the inherent issue of highly variable data, which can hinder interpretation. We introduced a simple and robust sampling methodology based on silicone rod extraction, thermal desorption gas chromatography - mass spectrometry (GC-MS) to address these issues. We used differentiated calibration standards to generate quantitative data for metabolites of varying abundance. The method was used to profile 327 melons with high sensitivity (0.05-10?ª+ng/mL, compound dependent), good reproducibility (7%) and differentiate melon varieties based on their volatile profile. The data were then used for line selection for a desired flavor profile"
Keywords:		Cucurbitaceae/*chemistry Gas Chromatography-Mass Spectrometry *Plant Breeding Reproducibility of Results Silicones Volatile Organic Compounds/*analysis Flavor profiling Gc-ms Line selection Marker assisted breeding Melon Plant breeding Silicone rod extrac;
Notes:		"MedlineKende, Aniko Lim, Pei Pei Lai, Florence Jessop, Michael Swindale, Lauraine Oliver, Marc Hurr, Brandon Rickett, Daniel Baxter, Charles eng England 2018/09/04 Food Chem. 2019 Jan 1; 270:368-374. doi: 10.1016/j.foodchem.2018.07.101. Epub 2018 Jul 17"