| Title: | Molecular characterization of a moss isoprene synthase provides insight into its evolution |
| Author(s): | Kawakami T; Miyazaki S; Kawaide H; |
| Address: | "Graduate School of Agriculture, Tokyo University of Agriculture and Technology (TUAT), Fuchu, Japan. Institute of Global Innovation Research, Tokyo University of Agriculture and Technology (TUAT), Fuchu, Japan" |
| ISSN/ISBN: | 1873-3468 (Electronic) 0014-5793 (Linking) |
| Abstract: | "This is the first report on the molecular characterization of isoprene synthase (ISPS) from the moss Calohypnum plumiforme. After isoprene emission from C. plumiforme was confirmed, the cDNA encoding C. plumiforme ISPS (CpISPS) was narrowed down using a genome database associated with protein structure prediction, and a CpISPS gene was identified. The recombinant CpISPS, produced in Escherichia coli, converted dimethylallyl diphosphate to isoprene. Phylogenetic analysis indicated similarity between the amino acid sequences of CpISPS and moss diterpene cyclases (DTCs) but not ISPSs of higher plants, implying that CpISPS is derived from moss DTCs and is evolutionarily unrelated to canonical ISPSs of higher plants. CpISPS is a novel class I cyclase of the terpene synthase-c subfamily harboring alphabeta domains. This study will help further study of isoprene biosynthesis and the physiological functions of isoprene in mosses" |
| Keywords: | "Phylogeny Plant Proteins/genetics/metabolism *Alkyl and Aryl Transferases/genetics *Bryophyta/genetics/metabolism Butadienes *Diterpenes Evolution, Molecular Physcomitrium patens bryophyte copalyl diphosphate isoprene synthase volatile organic compounds;" |
| Notes: | "MedlineKawakami, Tetsuya Miyazaki, Sho Kawaide, Hiroshi eng Research Support, Non-U.S. Gov't England 2023/06/30 FEBS Lett. 2023 Aug; 597(16):2133-2142. doi: 10.1002/1873-3468.14691. Epub 2023 Jul 4" |
