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Anal Chem

Title:		"Fiber-Sample Distance, An Important Parameter To Be Considered in Headspace Solid-Phase Microextraction Applications"
Author(s):		Kamgang Nzekoue F; Angeloni S; Caprioli G; Cortese M; Maggi F; Marconi UMB; Perali A; Ricciutelli M; Sagratini G; Vittori S;
Address:		"School of Sciences and Technology, University of Camerino, Via Madonna delle Carceri, 62032 Camerino, Italy"
Journal Title:		Anal Chem
Year:		2020
Volume:		20200520
Issue:		11
Page Number:		7478 - 7484
DOI:		10.1021/acs.analchem.9b05386
ISSN/ISBN:		1520-6882 (Electronic) 0003-2700 (Print) 0003-2700 (Linking)
Abstract:		"To define and control the parameters which impact headspace solid-phase microextraction (HS-SPME), it is important to reach the highest level of reproducibility. The present study aims to assess, for the first time, the effect of fiber-sample distance during HS-SPME in pre-equilibrium conditions. Analyses were primarily performed on mixtures of standard volatiles compounds (alkanes, alcohols, organic acids) designed in our lab and then on various food matrices (wine, chicken, cheese, tea), repeating already published experiments. Extractions were performed varying fiber penetration depths (10-60 mm) at different times (10-60 min) and temperatures of extraction (30-80 degrees C). The study revealed that variation of the distance between the fiber and the sample into the vial clearly impacts the results obtained during HS-SPME when conditions are such that no equilibrium is reached in HS. For example, in wine analysis, the percentage of octanoic acid at 80 degrees C was higher at 40 mm (7.5 +/- 0.2%) than that at 20 mm (4.4 +/- 0.3%). Moreover, regardless of the extraction temperature, the lower the time of extraction, the stronger the dependence on the fiber-sample distance. Indeed, at 60 degrees C, the obtained response factors for octadecane at 20 and 40 mm of fiber penetration were 21.8 and 44.5, respectively, after 10 min of extraction, 54.1 and 71.0 after 30 min, and 79.4 and 82.4 after 60 min of extraction. The analyses have been here corroborated by a theoretical model based on the diffusion equation. Therefore, to improve the method robustness during HS-SPME studies, we suggest specifying the fiber penetration depth or the fiber-sample distance with the other parameters of extraction"
Keywords:
Notes:		"PubMed-not-MEDLINEKamgang Nzekoue, Franks Angeloni, Simone Caprioli, Giovanni Cortese, Manuela Maggi, Filippo Marconi, Umberto Marini Bettolo Perali, Andrea Ricciutelli, Massimo Sagratini, Gianni Vittori, Sauro eng 2020/05/10 Anal Chem. 2020 Jun 2; 92(11):7478-7484. doi: 10.1021/acs.analchem.9b05386. Epub 2020 May 20"