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Biotechnol Bioeng

Title:		"Improvement of surfactin production in Bacillus subtilis using synthetic wastewater by overexpression of specific extracellular signaling peptides, comX and phrC"
Author(s):		Jung J; Yu KO; Ramzi AB; Choe SH; Kim SW; Han SO;
Address:		"School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea"
Journal Title:		Biotechnol Bioeng
Year:		2012
Volume:		20120424
Issue:		9
Page Number:		2349 - 2356
DOI:		10.1002/bit.24524
ISSN/ISBN:		1097-0290 (Electronic) 0006-3592 (Linking)
Abstract:		"Surfactin is a biological surfactant with numerous potential applications. In this study, Bacillus subtilis was engineered to improve surfactin production by the activation of two competence-stimulating pheromones, ComX and competence and sporulation factor (CSF) to stimulate the transcription of srfA operon. Both signaling factors, encoded by comX and phrC, were successfully overexpressed and subsequently increased surfactin production. Surfactin produced by engineered strains showed functional groups similar to the commercially available surfactin analyzed via Fourier transform infrared spectroscopy (FTIR). Surfactin production in the B. subtilis (pHT43-comXphrC) strain was 6.4-fold greater than in the wild strain, with approximately 135.1 mg/L surfactin produced after 48 h cultivation. To reduce the production costs of surfactin, synthetic wastewater was used, from which the B. subtilis (pHT43-comXphrC) strain produced approximately 140.2 mg/L surfactin. The results obtained demonstrated the production of surfactin from synthetic wastewater, which is beneficial in lowering the overall production costs"
Keywords:		"Bacillus subtilis/genetics/*metabolism Bacterial Proteins/*biosynthesis/genetics/metabolism Gene Expression Regulation, Bacterial Genetic Engineering Lipopeptides/*biosynthesis/economics/metabolism Models, Chemical Peptides, Cyclic/*biosynthesis/economics;"
Notes:		"MedlineJung, Ju Yu, Kyung Ok Ramzi, Ahmad Bazli Choe, Se Hoon Kim, Seung Wook Han, Sung Ok eng Research Support, Non-U.S. Gov't 2012/04/19 Biotechnol Bioeng. 2012 Sep; 109(9):2349-56. doi: 10.1002/bit.24524. Epub 2012 Apr 24"