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Insect Biochem Mol Biol

Title:		Down-regulation of lysosomal protein ABCB6 increases gossypol susceptibility in Helicoverpa armigera
Author(s):		Jin M; Cheng Y; Guo X; Li M; Chakrabarty S; Liu K; Wu K; Xiao Y;
Address:		"Guangdong Laboratory of Lingnan Modern Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. Guangdong Laboratory of Lingnan Modern Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China. School of Life Sciences, Central China Normal University, Wuhan, 430079, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. Guangdong Laboratory of Lingnan Modern Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China. Electronic address: xiaoyutao@caas.cn"
Journal Title:		Insect Biochem Mol Biol
Year:		2020
Volume:		20200429
Issue:
Page Number:		103387 -
DOI:		10.1016/j.ibmb.2020.103387
ISSN/ISBN:		1879-0240 (Electronic) 0965-1748 (Linking)
Abstract:		"Cotton bollworm (Helicoverpa armigera) is the major insect herbivore of cotton plants. As its larvae feed and grow on cotton, H. armigera can likely tolerate gossypol, the main defense metabolite produced by cotton plants, through detoxification and sequestration mechanisms. Recent reports have shown that various P450 monooxygenases and UDP-glycosyltransferases in H. armigera are involved in gossypol detoxification, while the roles of ABC transporters, another gene family widely associated with metabolite detoxification, remain to be elucidated. Here, we show that ingestion of gossypol-infused artificial diet and cotton leaves significantly induced the expression of HaABCB6 in H. armigera larvae. Knockdown and knockout of HaABCB6 increased sensitivity of H. armigera larvae to gossypol. Moreover, HaABCB6-GFP fusion protein was localized on lysosomes in Hi5 cells and its overexpression significantly enhanced gossypol tolerance in vitro. These experimental results strongly support that HaABCB6 plays an important role in gossypol detoxification by H. armigera"
Keywords:		"ATP Binding Cassette Transporter, Subfamily B/*genetics/metabolism Animals Down-Regulation Gossypol/*pharmacology Insect Proteins/*genetics/metabolism Insecticides/*pharmacology Larva/drug effects/growth & development/metabolism Moths/drug effects/*geneti;"
Notes:		"MedlineJin, Minghui Cheng, Ying Guo, Xueqin Li, Meizhi Chakrabarty, Swapan Liu, Kaiyu Wu, Kongming Xiao, Yutao eng Research Support, Non-U.S. Gov't England 2020/05/04 Insect Biochem Mol Biol. 2020 Jul; 122:103387. doi: 10.1016/j.ibmb.2020.103387. Epub 2020 Apr 29"