| Title: | "Isolation and functional characterization of the pheromone biosynthesis activating neuropeptide receptor of Chinese oak silkworm, Antheraea pernyi" |
| Author(s): | Jiang L; Zhang F; Hou Y; Thakur K; Hu F; Zhang JG; Jiang XF; Liu YQ; Wei ZJ; |
| Address: | "School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, PR China. Electronic address: lijiang@hfut.edu.cn. School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, PR China. School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, PR China. Electronic address: kumarikiran@hfut.edu.cn. School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, PR China. Electronic address: hufei@hfut.edu.cn. School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, PR China. Electronic address: zhangjianguo@hfut.edu.cn. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China. Electronic address: xfjiang@ippcaas.cn. College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, PR China. Electronic address: liuyanqun@syau.edu.cn. School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, PR China. Electronic address: zjwei@hfut.edu.cn" |
| DOI: | 10.1016/j.ijbiomac.2018.05.145 |
| ISSN/ISBN: | 1879-0003 (Electronic) 0141-8130 (Linking) |
| Abstract: | "Insect pheromone biosynthesis activating neuropeptide (PBAN) controls the synthesis and actuating of sex pheromones of female adult. In the current examination, the full-length cDNA encoding the PBAN receptor was cloned from the pheromone gland (PG) of Antheraea pernyi (AntpePBANR). The AntpePBANR displayed the characteristic seven transmembrane areas of the G protein-coupled receptor (GPCR) and was closely related to the PBANR from Bombyx mori and Manduca sexta in the phylogenetic tree. The AntpePBANR expressed in mammalian cell lines were enacted by AntpePBAN in a concentration-dependent manner. AntpePBANR activation resulted in the calcium mobilization but did not activate the cAMP elevation pathway. Cells expressing AntpePBANR were profoundly responsive to Antpe-gamma-SGNP (suboesophageal ganglion neuropeptides) and Antpe-DH (diapause hormone), different individuals from FXPRLamide (X?ª+=?ª+T, S or V) family in A. pernyi. Deletion of residues in the C-terminal hexapeptide (FSPRLamide) proved that P, R and L played the key parts in initiating the AntpePBANR, the amination to the last C terminal residues which can also likewise impact the activation of AntpePBAN receptor altogether. The mRNA of the AntpePBANR gene demonstrated the most noteworthy transcript levels in pheromone gland followed by fat body" |
| Keywords: | "Amino Acid Sequence Animals Base Sequence Bombyx/classification/*genetics/*metabolism Calcium/metabolism Cell Line Cloning, Molecular DNA, Complementary Gene Expression Humans Neuropeptides/chemistry/*genetics/*metabolism Pheromones/metabolism Phylogeny R;" |
| Notes: | "MedlineJiang, Li Zhang, Fang Hou, Yang Thakur, Kiran Hu, Fei Zhang, Jian-Guo Jiang, Xing-Fu Liu, Yan-Qun Wei, Zhao-Jun eng Netherlands 2018/05/26 Int J Biol Macromol. 2018 Oct 1; 117:42-50. doi: 10.1016/j.ijbiomac.2018.05.145. Epub 2018 May 23" |
