| Title: | Allyl isothiocyanate affects the cell cycle of Arabidopsis thaliana |
| Author(s): | Asberg SE; Bones AM; Overby A; |
| Address: | "Department of Biology, Norwegian University of Science and Technology Trondheim, Norway" |
| ISSN/ISBN: | 1664-462X (Print) 1664-462X (Electronic) 1664-462X (Linking) |
| Abstract: | "Isothiocyanates (ITCs) are degradation products of glucosinolates present in members of the Brassicaceae family acting as herbivore repellents and antimicrobial compounds. Recent results indicate that allyl ITC (AITC) has a role in defense responses such as glutathione depletion, ROS generation and stomatal closure. In this study we show that exposure to non-lethal concentrations of AITC causes a shift in the cell cycle distribution of Arabidopsis thaliana leading to accumulation of cells in S-phases and a reduced number of cells in non-replicating phases. Furthermore, transcriptional analysis revealed an AITC-induced up-regulation of the gene encoding cyclin-dependent kinase A while several genes encoding mitotic proteins were down-regulated, suggesting an inhibition of mitotic processes. Interestingly, visualization of DNA synthesis indicated that exposure to AITC reduced the rate of DNA replication. Taken together, these results indicate that non-lethal concentrations of AITC induce cells of A. thaliana to enter the cell cycle and accumulate in S-phases, presumably as a part of a defensive response. Thus, this study suggests that AITC has several roles in plant defense and add evidence to the growing data supporting a multifunctional role of glucosinolates and their degradation products in plants" |
| Keywords: | Allyl isothiocyanate Arabidopsis thaliana cell cycle isothiocyanates plant defense; |
| Notes: | "PubMed-not-MEDLINEAsberg, Signe E Bones, Atle M Overby, Anders eng Switzerland 2015/06/05 Front Plant Sci. 2015 May 19; 6:364. doi: 10.3389/fpls.2015.00364. eCollection 2015" |
