| Title: | Cadmium exposure during prenatal development causes testosterone disruption in multigeneration via SF-1 signaling in rats |
| Author(s): | Huang Y; Zhu J; Li H; Wang W; Li Y; Yang X; Zheng N; Liu Q; Zhang Q; Zhang W; Liu J; |
| Address: | "Department of Preventive Medicine, Fujian Provincial Key Laboratory of Environmental Factors and Cancer, Key Laboratory of Environment and Health, School of Public Health, Fujian Medical University, Xueyan Road No. 1, Minhou County, Fuzhou, 350108, China. Fujian Health College Heath Management Faculty, Minhou County, Fuzhou, 350108, China. Department of Health Inspection and Quarantine, School of Public Health, Fujian Medical University, Xueyan Road No. 1, Minhou County, Fuzhou, 350108, China. Department of Environmental and Occupational Health Sciences, School of Public Health and Information Sciences, University of Louisville, 485 E. Gray Street, Louisville, KY, 40202, USA. Department of Preventive Medicine, Fujian Provincial Key Laboratory of Environmental Factors and Cancer, Key Laboratory of Environment and Health, School of Public Health, Fujian Medical University, Xueyan Road No. 1, Minhou County, Fuzhou, 350108, China. Electronic address: liujin@fjmu.edu.cn" |
| DOI: | 10.1016/j.fct.2019.110897 |
| ISSN/ISBN: | 1873-6351 (Electronic) 0278-6915 (Linking) |
| Abstract: | "The mechanisms of prenatal cadmium (Cd) exposure cause adverse effect transmission to future generations that remain unclear. In this study, pregnant SD rats were orally dosed with Cd (0, 0.5, 2.0, and 8.0?ª+mg/kg/day) from gestation day 1 until birth. F1 female rats were mated with untreated males for F2 generation. In both generations, after prenatal Cd exposure, histopathological examinations showed testicular development disorder. A significant decrease in serum testosterone (T) was observed in the F1 rats, but a significant increase in serum T was observed in the F2 rats. Moreover, both the F1 and F2 rats had different patterns of mRNA and protein expression for testicular steroidogenic factor 1 (SF-1) and steroidogenic enzymes at postnatal days (PNDs) 21 and 56. We also found that rno-miR-328a-5 and rno-miR-10b-5p significantly changed and TargetScan software showed that both of these microRNAs targeted SF-1 and steroidogenic acute regulatory (StAR), respectively. Overall, the results indicate that prenatal Cd exposure causes male reproductive problems in a multigenerational manner. In addition, SF-1 signaling was disrupted and the expressions of microRNAs were affected, which may be an important target for Cd-induced reproductive toxicity in offspring" |
| Keywords: | "Animals Cadmium/*toxicity Female Gonadotropin-Releasing Hormone/metabolism Luteinizing Hormone/metabolism Male Maternal Exposure/adverse effects MicroRNAs/metabolism Pregnancy Prenatal Exposure Delayed Effects/*metabolism Progesterone/metabolism Rats, Spr;" |
| Notes: | "MedlineHuang, Yuanyuan Zhu, Jianlin Li, Hong Wang, Wenxiang Li, Yuchen Yang, Xiao Zheng, Nahong Liu, Qiumei Zhang, Qunwei Zhang, Wenchang Liu, Jin eng England 2019/10/28 Food Chem Toxicol. 2020 Jan; 135:110897. doi: 10.1016/j.fct.2019.110897. Epub 2019 Oct 22" |
