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Fungal Biol

Title:		"Cytochrome P450 complement (CYPome) of Candida oregonensis, a gut-associated yeast of bark beetle, Dendroctonus rhizophagus"
Author(s):		Hernandez-Martinez F; Briones-Roblero CI; Nelson DR; Rivera-Orduna FN; Zuniga G;
Address:		"Departamento de Zoologia, Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional, Prolongacion de Carpio y Plan de Ayala, Col. Sto. Tomas, Mexico D.F. CP 11340, Mexico. Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, 858 Madison Ave. Suite G01, Memphis, TN 38163, USA. Departamento de Microbiologia, Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional, Prolongacion de Carpio y Plan de Ayala, Col. Sto. Tomas, Mexico D.F. CP 11340, Mexico. Departamento de Zoologia, Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional, Prolongacion de Carpio y Plan de Ayala, Col. Sto. Tomas, Mexico D.F. CP 11340, Mexico. Electronic address: capotezu@hotmail.com"
Journal Title:		Fungal Biol
Year:		2016
Volume:		20160617
Issue:		9
Page Number:		1077 - 1089
DOI:		10.1016/j.funbio.2016.06.005
ISSN/ISBN:		1878-6146 (Print)
Abstract:		"Bark beetles (Curculionidae: Scolytinae) and associated microorganisms must overcome a complex tree's defence system, which includes toxic monoterpenes, to successfully complete their life cycle. A number of studies have suggested these microorganisms could have ecological roles related with the nutrition, detoxification, and semiochemical production. In particular, in filamentous fungi symbionts, cytochrome P450 (CYP) have been involved with terpenoid detoxification and biotransformation processes. Candida oregonensis has been isolated from the gut, ovaries, and frass of different bark beetle species, and it is a dominant species in the Dendroctonus rhizophagus gut. In this study, we identify, characterise, and infer the phylogenetic relationships of C. oregonensis CYP genes. The results indicate that the cytochrome P450 complement (CYPome) is composed of nine genes (CYP51F1, CYP61A1, CYP56D1, CYP52A59, CYP52A60, CYP52A61, CYP52A62, CYP5217A8, and CYP5217B1), which might participate in primary metabolic reactions such as sterol biosynthesis, biodegradation of xenobiotic, and resistance to environmental stress. The prediction of the cellular location suggests that these CYPs to be anchored to the plasma membrane, membranes of the endoplasmic reticulum, mitochondria, and peroxisomes. These findings lay the foundation for future studies about the functional role of P450s, not only for yeasts, but also for the insects with which they interact"
Keywords:		Animals Candida/*classification/*enzymology/genetics/isolation & purification Cell Membrane/enzymology Cytochrome P-450 Enzyme System/*genetics Gastrointestinal Tract/microbiology Intracellular Membranes/enzymology *Phylogeny Sequence Homology Weevils/*mi;
Notes:		"MedlineHernandez-Martinez, Fabiola Briones-Roblero, Carlos Ivan Nelson, David R Rivera-Orduna, Flor Nohemi Zuniga, Gerardo eng Netherlands 2016/08/29 Fungal Biol. 2016 Sep; 120(9):1077-89. doi: 10.1016/j.funbio.2016.06.005. Epub 2016 Jun 17"