| Title: | Ligand recognition in multiallelic pheromone receptors from the basidiomycete Schizophyllum commune studied in yeast |
| Author(s): | Hegner J; Siebert-Bartholmei C; Kothe E; |
| Address: | "Department of Biology-Genetics, Philipps University, Marburg, 35032, Germany" |
| ISSN/ISBN: | 1087-1845 (Print) 1087-1845 (Linking) |
| Abstract: | "The homobasidiomycete Schizophyllum commune encodes a multiallelic pheromone receptor system that distinguishes more than 20 nonself from at least 2 self pheromones. The well-investigated pheromone response system of the yeast Saccharomyces cerevisiae was used to link the FUS1::lacZ reporter system to the heterologous pheromone receptors from S. commune. To investigate yeast G-protein binding, the unchanged heterologous receptor was compared to constructs carrying an exchange of the 3rd cytoplasmatic loop for the Ste2 sequence. A better coupling could be achieved with the altered constructs. In order to examine activation by single pheromones, an artificial peptide based on the sequence of a new putative pheromone gene, bap2(1), in the Balpha2 mating-type locus encoding the shortest pheromone found so far in fungal mating types was used. Thus, we have reassembled the pheromone recognition of the basidiomycete S. commune and constructed a system ideal for specificity analysis in the yeast S. cerevisiae" |
| Keywords: | "Amino Acid Sequence Base Sequence Chemoreceptor Cells/*metabolism *Genes, Fungal *Genes, Mating Type, Fungal Genes, Reporter/genetics Ligands Molecular Sequence Data Schizophyllum/chemistry/genetics/*metabolism Sequence Homology, Nucleic Acid Transfection;" |
| Notes: | "MedlineHegner, J Siebert-Bartholmei, C Kothe, E eng 1999/06/11 Fungal Genet Biol. 1999 Apr; 26(3):190-7. doi: 10.1006/fgbi.1999.1119" |
