| Title: | "A Bacterial Quorum-Sensing Precursor Induces Mortality in the Marine Coccolithophore, Emiliania huxleyi" |
| Author(s): | Harvey EL; Deering RW; Rowley DC; El Gamal A; Schorn M; Moore BS; Johnson MD; Mincer TJ; Whalen KE; |
| Address: | "Department of Marine Sciences, Skidaway Institute of Oceanography, University of Georgia, Savannah GA, USA. Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island, Kingston RI, USA. Scripps Institution of Oceanography, University of California at San Diego, La Jolla CA, USA. Biology Department, Woods Hole Oceanographic Institution, Woods Hole MA, USA. Marine Chemistry and Geochemistry Department, Woods Hole Oceanographic Institution, Woods Hole MA, USA" |
| ISSN/ISBN: | 1664-302X (Print) 1664-302X (Electronic) 1664-302X (Linking) |
| Abstract: | "Interactions between phytoplankton and bacteria play a central role in mediating biogeochemical cycling and food web structure in the ocean. However, deciphering the chemical drivers of these interspecies interactions remains challenging. Here, we report the isolation of 2-heptyl-4-quinolone (HHQ), released by Pseudoalteromonas piscicida, a marine gamma-proteobacteria previously reported to induce phytoplankton mortality through a hitherto unknown algicidal mechanism. HHQ functions as both an antibiotic and a bacterial signaling molecule in cell-cell communication in clinical infection models. Co-culture of the bloom-forming coccolithophore, Emiliania huxleyi with both live P. piscicida and cell-free filtrates caused a significant decrease in algal growth. Investigations of the P. piscicida exometabolome revealed HHQ, at nanomolar concentrations, induced mortality in three strains of E. huxleyi. Mortality of E. huxleyi in response to HHQ occurred slowly, implying static growth rather than a singular loss event (e.g., rapid cell lysis). In contrast, the marine chlorophyte, Dunaliella tertiolecta and diatom, Phaeodactylum tricornutum were unaffected by HHQ exposures. These results suggest that HHQ mediates the type of inter-domain interactions that cause shifts in phytoplankton population dynamics. These chemically mediated interactions, and other like it, ultimately influence large-scale oceanographic processes" |
| Keywords: | Emiliania huxleyi Hhq Ic50 Pseudoalteromonas algicidal compound bacteria-phytoplankton interaction infochemicals mortality; |
| Notes: | "PubMed-not-MEDLINEHarvey, Elizabeth L Deering, Robert W Rowley, David C El Gamal, Abrahim Schorn, Michelle Moore, Bradley S Johnson, Matthew D Mincer, Tracy J Whalen, Kristen E eng P01 ES021921/ES/NIEHS NIH HHS/ P20 GM103430/GM/NIGMS NIH HHS/ Switzerland 2016/02/13 Front Microbiol. 2016 Feb 3; 7:59. doi: 10.3389/fmicb.2016.00059. eCollection 2016" |
