| Title: | Functional differentiation of the ovine preadipocytes -insights from gene expression profiling |
| Author(s): | Hao Z; Jin X; Wang J; Luo Y; Hu J; Liu X; Li S; Zhao F; Li M; |
| Address: | "Gansu Key Laboratory of Herbivorous Animal Biotechnology, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China. Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining, China. Gansu Key Laboratory of Herbivorous Animal Biotechnology, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China. wangjq@gsau.edu.cn" |
| DOI: | 10.1007/s10142-023-01034-y |
| ISSN/ISBN: | 1438-7948 (Electronic) 1438-793X (Linking) |
| Abstract: | "The preadipocytes differentiation is a vital process of lipogenesis; exploring the molecular mechanisms of lipogenesis contributes to improve the meat quality and final commercial income. Lipogenesis has been widely reported in other livestock, but little is known about the gene expression profiles at different stages during preadipocytes differentiation in sheep. In this study, ovine preadipocytes were cultured in vitro and then induced to begin differentiation. Then, the gene expression profiles of preadipocytes collected on day 0 (D0), day 2 (D2), and day 8 (D8) of differentiation were analyzed by RNA-seq technology. According to the findings, 2254 differentially expressed genes (DEGs) were found in D2 vs D0; 1817 DEGs and 1902 DEGs were found in D8 vs D0 and D8 vs D2, respectively. The DEGs were found to be enriched in several biological processes, including focal adhesion, ECM-receptor interaction, PI3K-Akt signaling pathway, steroid biosynthesis, and MAPK signaling pathway, according to Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The regulatory network of the DEGs related to ovine preadipocytes differentiation was systematically constructed, which showed that hub genes might modulate ovine preadipocytes differentiation. In summary, preadipocyte differentiation is regulated by several key genes, including ACACB, CXCL6, SREBF1, INSIG1, APOE, GJA1, CDH11, SYNE1, PCSK1, S100A4, FN1, PLIN2, CXCL6, FN1, PTX3, and FABP3. This study provides a deeper knowledge of the roles of genes in sheep lipogenesis by revealing global gene expression profiles during preadipocyte differentiation" |
| Keywords: | Animals Sheep/genetics *Phosphatidylinositol 3-Kinases *Gene Expression Profiling Transcriptome Microarray Analysis RNA-Seq Gene Regulatory Networks Computational Biology Gene Ontology Differentiation Gene expression Preadipocytes Sheep; |
| Notes: | "MedlineHao, Zhiyun Jin, Xiayang Wang, Jiqing Luo, Yuzhu Hu, Jiang Liu, Xiu Li, Shaobin Zhao, Fangfang Li, Mingna eng GAU-DK-QNJJ-202102/The Youth Fund of College of Animal Science and Technology of Gansu Agricultural University/ Germany 2023/03/24 Funct Integr Genomics. 2023 Mar 23; 23(2):97. doi: 10.1007/s10142-023-01034-y" |
