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FEBS Lett

Title:		Expression of highly active sex-inducing pheromone of Volvox carteri f. nagariensis in a mammalian cell system
Author(s):		Jaenicke L; Feldwisch O; Merkl B; Cremer A; Haas IG;
Address:		"Institute of Biochemistry, University of Cologne, Germany"
Journal Title:		FEBS Lett
Year:		1993
Volume:		316
Issue:		3
Page Number:		257 - 260
DOI:		10.1016/0014-5793(93)81303-h
ISSN/ISBN:		0014-5793 (Print) 0014-5793 (Linking)
Abstract:		"A cDNA fragment coding for the sex-inducing glycoprotein of Volvox carteri f. nagariensis was expressed in a mammalian cell system (baby hamster kidney (BHK) cells). The transfection product exhibited a specific biological activity intermediate between the natural pheromone of the strains Volvox carteri f. nagariensis and Volvox carteri f. weismannia. Immunoblot analysis showed that the sex-inducing activity was expressed as a set of three iso-glycoproteins (35, 34 and 31 kDa)"
Keywords:		"Cells, Cultured Chlorophyta/*genetics/physiology Cloning, Molecular DNA/genetics Glycoproteins/genetics In Vitro Techniques Molecular Weight Sex Attractants/*genetics Transfection;"
Notes:		"MedlineJaenicke, L Feldwisch, O Merkl, B Cremer, A Haas, I G eng Research Support, Non-U.S. Gov't England 1993/02/01 FEBS Lett. 1993 Feb 1; 316(3):257-60. doi: 10.1016/0014-5793(93)81303-h"