| Title: | Comparative Gene Expression and Physiological Analyses Reveal Molecular Mechanisms in Wound-Induced Spore Formation in the Edible Seaweed Nori |
| Author(s): | Guan X; Mao Y; Stiller JW; Shu S; Pang Y; Qu W; Zhang Z; Tang F; Qian H; Chen R; Sun B; Guoying D; Mo Z; Kong F; Tang X; Wang D; |
| Address: | "Key Laboratory of Marine Genetics and Breeding (OUC), Ministry of Education, Qingdao, China. College of Marine Life Sciences, Ocean University of China, Qingdao, China. Key Laboratory of Utilization and Conservation for Tropical Marine Bioresources (Hainan Tropical Ocean University), Ministry of Education, Sanya, China. Department of Biology, East Carolina University, Greenville, NC, United States" |
| ISSN/ISBN: | 1664-462X (Print) 1664-462X (Electronic) 1664-462X (Linking) |
| Abstract: | "Genetic reprogramming of differentiated cells is studied broadly in multicellular Viridiplantae as an adaptation to herbivory or damage; however, mechanisms underlying cell development and redifferentiation are largely unknown in red algae, their nearest multicellular relatives. Here we investgate cell reprogramming in the widely cultivated, edible seaweed Neopyropia yezoesis ('nori'), where vegetative cells in wounded blades differentiate and release as large numbers of asexual spores. Based upon physiological changes and transcriptomic dynamics after wound stress in N. yezoensis and its congener Neoporphyra haitanensis, another cultivar that does not differentiate spores after wounding, we propose a three-phase model of wound-induced spore development in N. yezoensis. In Phase I, propagation of ROS by RBOH and SOD elicites systematic transduction of the wound signal, while Ca(2+) dependent signaling induces cell reprogramming. In Phase II, a TOR signaling pathway and regulation of cyclin and CDK genes result in cell divisions that spread inward from the wound edge. Once sporangia form, Phase III involves expression of proteins required for spore maturation and cell wall softening. Our analyses not only provide the first model for core molecular processes controlling cellular reprogramming in rhodophytes, but also have practical implications for achieving greater control over seeding in commercial nori farming" |
| Keywords: | Neopyropia yezoensis Ros calcium signaling pathway cell division cell reprogramming nori spore-specific gene marker wound-induced spores; |
| Notes: | "PubMed-not-MEDLINEGuan, Xiaowei Mao, Yunxiang Stiller, John W Shu, Shanshan Pang, Ying Qu, Weihua Zhang, Zehao Tang, Fugeng Qian, Huijuan Chen, Rui Sun, Bin Guoying, Du Mo, Zhaolan Kong, Fanna Tang, Xianghai Wang, Dongmei eng Switzerland 2022/04/05 Front Plant Sci. 2022 Mar 17; 13:840439. doi: 10.3389/fpls.2022.840439. eCollection 2022" |
