| Title: | Determination of the reaction yield during biotransformation of the volatile and chemically unstable compound beta-ionone by aspergillus niger |
| Author(s): | Grivel F; Larroche C; Gros JB; |
| Address: | "Laboratoire de Genie Chimique Biologique, Universite Blaise Pascal, F-63177 Aubiere Cedex, France" |
| ISSN/ISBN: | 1520-6033 (Electronic) 1520-6033 (Linking) |
| Abstract: | "Biotransformation of beta-ionone by Aspergillus niger IFO 8541 was a complex system, due to the low precursor solubility in water (0.88 mol/m(3)) which gave a two-phase liquid system, its high volatility (vapor pressure 7.2 Pa), and its poor chemical stability. A dynamic model, considering transfers between organic, aqueous, and gas layers, was established. It also took into account the decrease in organic layer area exchange during a process, due to progressive precursor disappearance. Its solving needed separate determination of two parameters. The first-order kinetic constant for chemical degradation, K(1), was estimated from side experiments involving a model system at thermodynamic equilibrium; its value was found to be close to 4.17 x 10(-)(6) s(-)(1). The volumetric transfer coefficient between gas and aqueous phase was estimated by analogy with results obtained for oxygen transfer. Results obtained demonstrated high liquid-gas transfer rates, while liquid-liquid transfers were severely limited. These data were then used to estimate the true yield of a biotransformation experiment, which was found to be close to unity" |
| Notes: | "PublisherGrivel, F Larroche, C Gros, JB eng 1999/08/12 Biotechnol Prog. 1999 Jul; 15(4):697-705. doi: 10.1021/bp9900312" |
