| Title: | "Antennally mediated negative feedback regulation of pheromone production in the pine engraver beetle, Ips pini" |
| Author(s): | Ginzel MD; Bearfield JC; Keeling CI; McCormack CC; Blomquist GJ; Tittiger C; |
| Address: | "Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV 89557, USA. ginzel@unr.edu" |
| DOI: | 10.1007/s00114-006-0158-2 |
| ISSN/ISBN: | 0028-1042 (Print) 0028-1042 (Linking) |
| Abstract: | "Bark beetles use monoterpenoid aggregation pheromones to coordinate host colonization and mating. These chemical signals are produced de novo in midgut cells via the mevalonate pathway, and pheromone production may be regulated by a negative feedback system mediated through the antennae. In this study, we explored the effect of antennectomy on pheromone production and transcript levels of key mevalonate pathway genes in juvenile hormone III-treated male pine engraver beetles, Ips pini (Say). Antennectomized males produced significantly greater amounts of pheromone than podectomized males and those with intact antennae. Likewise, mRNA levels of three mevalonate pathway genes important in pheromone biosynthesis were measured by quantitative real-time PCR and found to be induced to a greater extent with antennectomy, suggesting a transcriptional regulation of pheromone production" |
| Keywords: | Animals Coleoptera/genetics/*physiology DNA Primers Feedback Gene Expression Regulation Pheromones/*physiology Pinus/*parasitology Plant Stems/parasitology Polymerase Chain Reaction; |
| Notes: | "MedlineGinzel, Matthew D Bearfield, Jeremy C Keeling, Christopher I McCormack, Colin C Blomquist, Gary J Tittiger, Claus eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Germany 2006/11/10 Naturwissenschaften. 2007 Jan; 94(1):61-4. doi: 10.1007/s00114-006-0158-2. Epub 2006 Nov 9" |
