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Anal Bioanal Chem

Title:		Distinguish oral-source VOCs and control their potential impact on breath biomarkers
Author(s):		Ge D; Zhou J; Chu Y; Lu Y; Zou X; Xia L; Liu Y; Huang C; Shen C; Zhang L; Wang H; Chu Y;
Address:		"Anhui Province Key Laboratory of Medical Physics and Technology, Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, Anhui, China. University of Science and Technology of China, Hefei, 230026, Anhui, China. Anhui Province Key Laboratory of Medical Physics and Technology, Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, Anhui, China. ylu@cmpt.ac.cn. Hefei Cancer Hospital, Chinese Academy of Sciences, Hefei, 230031, Anhui, China. ylu@cmpt.ac.cn. Hefei Cancer Hospital, Chinese Academy of Sciences, Hefei, 230031, Anhui, China. Anhui Province Key Laboratory of Medical Physics and Technology, Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, Anhui, China. ychu@aiofm.ac.cn"
Journal Title:		Anal Bioanal Chem
Year:		2022
Volume:		20220104
Issue:		6
Page Number:		2275 - 2284
DOI:		10.1007/s00216-021-03866-8
ISSN/ISBN:		1618-2650 (Electronic) 1618-2642 (Linking)
Abstract:		"By means of glass bottle sampling followed by solid-phase microextraction gas chromatography-mass spectrometry (SPME-GC-MS) technique, the change characteristics of volatile organic compounds (VOCs) in breaths, between before gargling and after gargling, were investigated, respectively, in 41 healthy subjects and 50 esophageal cancer patients. Using an untargeted strategy, 143 VOC chromatographic peaks were enrolled in the statistical analysis. Based on the orthogonal partial least squares discriminant analysis (OPLS-DA), the VOC variations after gargling for each breath test group were obtained according to the combined criteria of variable importance in projection (VIP > 1.5), Wilcoxon signed-rank test (P < 0.05), and fold change (FC > 2.0). When gargled, the levels of indole, phenol, 1-propanol, and p-cresol in the breath of healthy people decreased; meanwhile, for esophageal cancer patients, the declined VOCs in breath were indole, phenol, dimethyl disulfide, and p-cresol. Particularly, these substances were previously reported as breath biomarkers in some diseases such as esophageal, gastric, thyroid, breast, oral, and lung cancers, as well as certain non-cancer disorders. The present work indicates that expiratory VOCs involve the prominent oral cavity source, and in the breath biomarkers study, the potential impact that originates from oral volatiles should be considered. In view of the present results, it is also proposed that gargle pretreatment could eliminate possible interference from the oral cavity VOCs that might benefit breath biomarker investigation. Gargle pretreatment helps to distinguish oral-source VOCs and control their potential impact on breath biomarkers"
Keywords:		Biomarkers/analysis Breath Tests/methods Gas Chromatography-Mass Spectrometry/methods Humans Solid Phase Microextraction/methods *Volatile Organic Compounds/analysis Breath biomarker Gc-ms Gargling Oral cavity Untargeted analysis VOCs;
Notes:		"MedlineGe, Dianlong Zhou, Jijuan Chu, Yajing Lu, Yan Zou, Xue Xia, Lei Liu, Yawei Huang, Chaoqun Shen, Chengyin Zhang, Liwei Wang, Huanzhong Chu, Yannan eng Germany 2022/01/05 Anal Bioanal Chem. 2022 Mar; 414(6):2275-2284. doi: 10.1007/s00216-021-03866-8. Epub 2022 Jan 4"