| Title: | Mitochondrial DNA copy number and cytogenetic damage among fuel filling station attendants |
| Author(s): | Gaikwad AS; Mahmood R; Beerappa R; Karunamoorthy P; Venugopal D; |
| Address: | "ICMR-Regional Occupational Health Centre (Southern), Bangalore, India. Department of Biotechnology and Bioinformatics, Kuvempu University, Shimoga, India" |
| ISSN/ISBN: | 1098-2280 (Electronic) 0893-6692 (Linking) |
| Abstract: | "Fuel filling attendants are constantly exposed to the complex mixture of gasoline and all refinery environments are probably carcinogenic for humans. These workers are considered as an unorganized group in India and unaware of the risk. The present study was focused to monitor workplace pollutants (particulate matter size 10 [PM10 mum], total volatile organic compound [VOC], and carbon monoxide [CO]), benzene exposure (phenol), and to evaluate their genotoxicity effect with reference to relative mitochondrial DNA copy number (MtDNAcn), 8-OHdG (8-hydroxy-2'-deoxyguanosine), and micronuclei (MN) frequency (%) among fuel filling attendants. This study recorded 318 +/- 134 and 1,050 +/- 260 mug/m(3) time-weighted average concentration of PM10 and CO, respectively. However, total VOC levels recorded were below the detectable level (BDL) to 290 +/- 50 mug/m(3) . A total of 53 subjects (26 exposed and 27 control) participated in this study with similar sociodemographic information. It was noticed that fuel filling attendants were not using proper personal protective equipment (PPE) and are younger generation. The significantly (p = <.001) higher level of phenol, a metabolite of benzene, was detected in the exposed group. The significantly elevated level of urinary 8-OHdG (p = .01), MN frequency (p = .001), and relative MtDNAcn (p = .001) was observed in exposed group as compared to the control group. The study exemplify that workers were exposed to the benzene, workplace pollutant, and observed genotoxicity suggest malignancy risk. This study highlights the importance of biomonitoring in occupational settings to avoid malignancies. The possible engineering controls, frequent health check-ups, awareness about the risks, and PPE use can reduce health hazards" |
| Keywords: | "Case-Control Studies *Chromosome Aberrations *DNA Copy Number Variations DNA, Mitochondrial/*genetics Female *Gasoline Humans India Male *Occupational Exposure benzene biomonitoring genotoxicity particulate matter unorganized group workers volatile organi;" |
| Notes: | "MedlineGaikwad, Avinash S Mahmood, Riaz Beerappa, Ravichandran Karunamoorthy, Panjakumar Venugopal, Dhananjayan eng Indian Council of Medical Research-National Institute of Occupational Health, Ahmedabad,India/International Research Support, Non-U.S. Gov't 2020/08/21 Environ Mol Mutagen. 2020 Oct; 61(8):820-829. doi: 10.1002/em.22404. Epub 2020 Sep 2" |
