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Electrophoresis

Title:		Analysis of N-acylhomoserine lactones after alkaline hydrolysis and anion-exchange solid-phase extraction by capillary zone electrophoresis-mass spectrometry
Author(s):		Frommberger M; Hertkorn N; Englmann M; Jakoby S; Hartmann A; Kettrup A; Schmitt-Kopplin P;
Address:		"Institute of Ecological Chemistry, Rhizosphere Biology Group, GSF-Research Center for Environment and Health Neuherberg, Germany"
Journal Title:		Electrophoresis
Year:		2005
Volume:		26
Issue:		7-Aug
Page Number:		1523 - 1532
DOI:		10.1002/elps.200410365
ISSN/ISBN:		0173-0835 (Print) 0173-0835 (Linking)
Abstract:		"A quantitative, specific, and sensitive method for the determination of N-acylhomoserine lactones (HSLs - a group of bacterial semiochemicals) in the form of their hydrolysis products (N-acylhomoserines, HSs) is presented. Real samples were analyzed by capillary zone electrophoresis-mass spectrometry (CZE-MS) after alkaline lactonolysis and extraction by mixed-mode anion-exchange solid-phase extraction. The presented cleanup significantly speeds up the HSL extraction procedure, strongly reduces sample consumption, and is more selective compared to the commonly used liquid/liquid extraction. Completeness of the hydrolysis reaction was examined by nuclear magnetic resonance spectroscopy. This CZE-MS method complements recently published capillary separation techniques (nano liquid chromatography-MS, partial-filling micellar electrokinetic chromatography-MS, gas chromatography-MS) and provides a possibility to differentiate quantitatively between the homoserines (as naturally occurring degradation products) besides the intact homoserine lactones. The method was found to be quantitative down to a concentration of 0.05 microg/mL (limit of quantification), while the limit of detection was determined with 0.01 microg/mL - sufficient for the analysis of culture supernatants"
Keywords:		"4-Butyrolactone/*analogs & derivatives/analysis Alkalies/*chemistry Anion Exchange Resins Chromatography, Ion Exchange/instrumentation/*methods Electrophoresis, Capillary/*methods Hydrolysis Magnetic Resonance Spectroscopy Mass Spectrometry/*methods;"
Notes:		"MedlineFrommberger, Moritz Hertkorn, Norbert Englmann, Matthias Jakoby, Sarah Hartmann, Anton Kettrup, Antonius Schmitt-Kopplin, Philippe eng Germany 2005/03/19 Electrophoresis. 2005 Apr; 26(7-8):1523-32. doi: 10.1002/elps.200410365"