| Title: | Metabolite exchange between microbiome members produces compounds that influence Drosophila behavior |
| Author(s): | Fischer CN; Trautman EP; Crawford JM; Stabb EV; Handelsman J; Broderick NA; |
| Address: | "Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, United States. Department of Chemistry, Yale University, New Haven, United States. Department of Microbiology, University of Georgia, Athens, United States. Department of Molecular and Cell Biology, University of Connecticut, Storrs, United States. Institute for Systems Genomics, University of Connecticut, Storrs, United States" |
| ISSN/ISBN: | 2050-084X (Electronic) 2050-084X (Linking) |
| Abstract: | "Animals host multi-species microbial communities (microbiomes) whose properties may result from inter-species interactions; however, current understanding of host-microbiome interactions derives mostly from studies in which elucidation of microbe-microbe interactions is difficult. In exploring how Drosophila melanogaster acquires its microbiome, we found that a microbial community influences Drosophila olfactory and egg-laying behaviors differently than individual members. Drosophila prefers a Saccharomyces-Acetobacter co-culture to the same microorganisms grown individually and then mixed, a response mainly due to the conserved olfactory receptor, Or42b. Acetobacter metabolism of Saccharomyces-derived ethanol was necessary, and acetate and its metabolic derivatives were sufficient, for co-culture preference. Preference correlated with three emergent co-culture properties: ethanol catabolism, a distinct volatile profile, and yeast population decline. Egg-laying preference provided a context-dependent fitness benefit to larvae. We describe a molecular mechanism by which a microbial community affects animal behavior. Our results support a model whereby emergent metabolites signal a beneficial multispecies microbiome" |
| Keywords: | "Acetic Acid/metabolism Acetobacter/*metabolism Animals Behavior, Animal/*drug effects Drosophila melanogaster/*drug effects/*physiology Ethanol/metabolism *Microbiota Saccharomyces/*metabolism Volatile Organic Compounds/metabolism D.melanogaster S.cerev;" |
| Notes: | "MedlineFischer, Caleb N Trautman, Eric P Crawford, Jason M Stabb, Eric V Handelsman, Jo Broderick, Nichole A eng RC1 DK086831/DK/NIDDK NIH HHS/ UL1 TR001863/TR/NCATS NIH HHS/ T32 HG003198/HG/NHGRI NIH HHS/ R01 GM099563/GM/NIGMS NIH HHS/ T32 GM007499/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural England 2017/01/10 Elife. 2017 Jan 9; 6:e18855. doi: 10.7554/eLife.18855" |
