| Title: | Chemical Priming by Isothiocyanates Protects Against Intoxication by Products of the Mustard Oil Bomb |
| Author(s): | Ferber E; Gerhards J; Sauer M; Krischke M; Dittrich MT; Muller T; Berger S; Fekete A; Mueller MJ; |
| Address: | "Julius-von-Sachs-Institute of Biosciences, Biocenter, Pharmaceutical Biology, University of Wurzburg, Wurzburg, Germany. Department of Boinformatics, Biocenter, University of Wurzburg, Wurzburg, Germany. Institute of Clinical Biochemistry, University of Wurzburg, Wurzburg, Germany" |
| ISSN/ISBN: | 1664-462X (Print) 1664-462X (Electronic) 1664-462X (Linking) |
| Abstract: | "In Brassicaceae, tissue damage triggers the mustard oil bomb i.e., activates the degradation of glucosinolates by myrosinases leading to a rapid accumulation of isothiocyanates at the site of damage. Isothiocyanates are reactive electrophilic species (RES) known to covalently bind to thiols in proteins and glutathione, a process that is not only toxic to herbivores and microbes but can also cause cell death of healthy plant tissues. Previously, it has been shown that subtoxic isothiocyanate concentrations can induce transcriptional reprogramming in intact plant cells. Glutathione depletion by RES leading to breakdown of the redox potential has been proposed as a central and common RES signal transduction mechanism. Using transcriptome analyses, we show that after exposure of Arabidopsis seedlings (grown in liquid culture) to subtoxic concentrations of sulforaphane hundreds of genes were regulated without depletion of the cellular glutathione pool. Heat shock genes were among the most highly up-regulated genes and this response was found to be dependent on the canonical heat shock factors A1 (HSFA1). HSFA1-deficient plants were more sensitive to isothiocyanates than wild type plants. Moreover, pretreatment of Arabidopsis seedlings with subtoxic concentrations of isothiocyanates increased resistance against exposure to toxic levels of isothiocyanates and, hence, may reduce the autotoxicity of the mustard oil bomb by inducing cell protection mechanisms" |
| Keywords: | autotoxicity heat shock response isothiocyanates mustard oil bomb reactive electrophilic species redox homeostasis sulforaphane; |
| Notes: | "PubMed-not-MEDLINEFerber, Elena Gerhards, Julian Sauer, Miriam Krischke, Markus Dittrich, Marcus T Muller, Tobias Berger, Susanne Fekete, Agnes Mueller, Martin J eng Switzerland 2020/07/18 Front Plant Sci. 2020 Jun 26; 11:887. doi: 10.3389/fpls.2020.00887. eCollection 2020" |
