| Title: | Identification of volatile organic compounds in the urine of patients with cervical cancer. Test concept for timely screening |
| Author(s): | Diaz de Leon-Martinez L; Flores-Ramirez R; Lopez-Mendoza CM; Rodriguez-Aguilar M; Metha G; Zuniga-Martinez L; Ornelas-Rebolledo O; Alcantara-Quintana LE; |
| Address: | "Centro de Investigacion Aplicada en Ambiente y Salud (CIAAS), Avenida Sierra Leona No. 550, CP 78210, Colonia Lomas Segunda Seccion, San Luis Potosi, SLP, Mexico. Centro de Investigacion Aplicada en Ambiente y Salud (CIAAS), Avenida Sierra Leona No. 550, CP 78210, Colonia Lomas Segunda Seccion, San Luis Potosi, SLP, Mexico. Electronic address: rfloresra@conacyt.mx. Unidad de Innovacion en Diagnostico Celular y Molecular. Coordinacion para la Innovacion y la Aplicacion de la Ciencia y Tecnologia, Universidad Autonoma de San Luis Potosi, Av. Sierra Leona 550, Lomas 2a seccion, 78120 San Luis Potosi, Mexico. Universidad de Quintana Roo (UQROO), Quintana Roo, Mexico. CEO of Altus Lifescience, San Jose, CA, United States. Labinnova Center of Research in Breath for early diseases detection, Guadalajara, Mexico. Unidad de Innovacion en Diagnostico Celular y Molecular. Coordinacion para la Innovacion y la Aplicacion de la Ciencia y Tecnologia, Universidad Autonoma de San Luis Potosi, Av. Sierra Leona 550, Lomas 2a seccion, 78120 San Luis Potosi, Mexico. Electronic address: luz.alcantara@uaslp.mx" |
| DOI: | 10.1016/j.cca.2021.08.014 |
| ISSN/ISBN: | 1873-3492 (Electronic) 0009-8981 (Linking) |
| Abstract: | "The objective of this research was to identify a global chemical pattern of volatile organic compounds (VOCs) in urine capable of discriminating between women with cervical cancer (CC) and control women using an electronic nose and to elucidate potential biomarkers by gas chromatography-mass spectrometry (GC-MS). A cross-sectional study was performed, with 12 control women, 5 women with CIN (Cervical Intraepithelial Neoplasia) and 12 women with CC. Global VOCs in urine were assessed using an electronic nose and specific by GC-MS. Multivariate analysis was performed: Principal Component Analysis (PCA), Canonical Principal Coordinate Analysis (CAP) and Partial Least Squares Discriminant Analysis (PLS-DA) and the test's diagnostic power was evaluated through ROC (Receiver Operating Characteristic) curves. Results from the PCA between the control group compared to the CC present variability of 98.4% (PC(1) = 93.9%, PC(2) = 2.3% and PC(3) = 2.1%). CAP model shows a separation between the overall VOCs profile of the control and CC group with a correct classification of 94.7%. PLS-DA indicated that 8 sensors have a higher contribution in the CC group. The sensitivity, specificity, value reached 91.6% (61.5%-99.7%) and 100% (73.5%-100%) respectively, according to the ROC curve. GC-MS analysis indicated that 33 compounds occur only in the CC group and some of them have been found in other types of cancer. In all, this study provides the basis for the development of an accessible, non-invasive, sensitive and specific screening platform for cervical cancer through the application of electronic nose and chemometric analysis" |
| Keywords: | Cross-Sectional Studies Early Detection of Cancer Electronic Nose Female Humans *Uterine Cervical Neoplasms/diagnosis *Volatile Organic Compounds Cervical Cancer Chemoresistive sensors Human Papilloma Virus Screening test Volatile Organic Compounds; |
| Notes: | "MedlineDiaz de Leon-Martinez, Lorena Flores-Ramirez, Rogelio Lopez-Mendoza, Carlos Miguel Rodriguez-Aguilar, Maribel Metha, Garima Zuniga-Martinez, Lourdes Ornelas-Rebolledo, Omar Alcantara-Quintana, Luz Eugenia eng Netherlands 2021/08/22 Clin Chim Acta. 2021 Nov; 522:132-140. doi: 10.1016/j.cca.2021.08.014. Epub 2021 Aug 18" |
