| Title: | Breath Analysis: Comparison among Methodological Approaches for Breath Sampling |
| Author(s): | Di Gilio A; Palmisani J; Ventrella G; Facchini L; Catino A; Varesano N; Pizzutilo P; Galetta D; Borelli M; Barbieri P; Licen S; de Gennaro G; |
| Address: | "Department of Biology, University of Bari, 70126 Bari, Italy. Apulian Breath Analysis Center (CeRBA), Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Istituto Tumori 'Giovanni Paolo II', 70124 Bari, Italy. Thoracic Oncology Unit, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Istituto Tumori 'Giovanni Paolo II', 70124 Bari, Italy. Department of Chemical and Pharmaceutical Sciences, University of Trieste, 34127 Trieste, Italy" |
| DOI: | 10.3390/molecules25245823 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "Despite promising results obtained in the early diagnosis of several pathologies, breath analysis still remains an unused technique in clinical practice due to the lack of breath sampling standardized procedures able to guarantee a good repeatability and comparability of results. The most diffuse on an international scale breath sampling method uses polymeric bags, but, recently, devices named Mistral and ReCIVA, able to directly concentrate volatile organic compounds (VOCs) onto sorbent tubes, have been developed and launched on the market. In order to explore performances of these new automatic devices with respect to sampling in the polymeric bag and to study the differences in VOCs profile when whole or alveolar breath is collected and when pulmonary wash out with clean air is done, a tailored experimental design was developed. Three different breath sampling approaches were compared: (a) whole breath sampling by means of Tedlar bags, (b) the end-tidal breath collection using the Mistral sampler, and (c) the simultaneous collection of the whole and alveolar breath by using the ReCIVA. The obtained results showed that alveolar fraction of breath was relatively less affected by ambient air (AA) contaminants (p-values equal to 0.04 for Mistral and 0.002 for ReCIVA Low) with respect to whole breath (p-values equal to 0.97 for ReCIVA Whole). Compared to Tedlar bags, coherent results were obtained by using Mistral while lower VOCs levels were detected for samples (both breath and AA) collected by ReCIVA, likely due to uncorrected and fluctuating flow rates applied by this device. Finally, the analysis of all data also including data obtained by explorative analysis of the unique lung cancer (LC) breath sample showed that a clean air supply might determine a further confounding factor in breath analysis considering that lung wash-out is species-dependent" |
| Keywords: | Adult Breath Tests/instrumentation/*methods Data Analysis Female Gas Chromatography-Mass Spectrometry Humans Male Middle Aged Reproducibility of Results Research Design Specimen Handling Volatile Organic Compounds/analysis Young Adult Mistral ReCIVA breat; |
| Notes: | "MedlineDi Gilio, Alessia Palmisani, Jolanda Ventrella, Gianrocco Facchini, Laura Catino, Annamaria Varesano, Niccolo Pizzutilo, Pamela Galetta, Domenico Borelli, Massimo Barbieri, Pierluigi Licen, Sabina de Gennaro, Gianluigi eng Switzerland 2020/12/17 Molecules. 2020 Dec 10; 25(24):5823. doi: 10.3390/molecules25245823" |
