« Previous AbstractThe evolution of desaturase gene regulation involved in sex pheromone production in leafroller moths of the genus planotortrix    Next AbstractExhaled Volatile Organic Compounds during Inflammation Induced by TNF-alpha in Ventilated Rats »

J Breath Res

Title:		Influence of the respirator on volatile organic compounds: an animal study in rats over 24 hours
Author(s):		Albrecht FW; Huppe T; Fink T; Maurer F; Wolf A; Wolf B; Volk T; Baumbach JI; Kreuer S;
Address:		"Department of Anaesthesiology, Intensive Care and Pain Therapy, Saarland University Medical Center and Saarland University Faculty of Medicine, Building 57, D-66421 Homburg, Germany"
Journal Title:		J Breath Res
Year:		2015
Volume:		20150309
Issue:		1
Page Number:		16007 -
DOI:		10.1088/1752-7155/9/1/016007
ISSN/ISBN:		1752-7163 (Electronic) 1752-7155 (Linking)
Abstract:		"Long-term animal studies are needed to accomplish measurements of volatile organic compounds (VOCs) for medical diagnostics. In order to analyze the time course of VOCs, it is necessary to ventilate these animals. Therefore, a total of 10 male Sprague-Dawley rats were anaesthetized and ventilated with synthetic air via tracheotomy for 24 h. An ion mobility spectrometry coupled to multi-capillary columns (MCC-IMS) was used to analyze the expired air. To identify background contaminations produced by the respirator itself, six comparative measurements were conducted with ventilators only. Overall, a number of 37 peaks could be detected within the positive mode. According to the ratio peak intensity rat/ peak intensity ventilator blank, 22 peaks with a ratio >1.5 were defined as expired VOCs, 12 peaks with a ratio between 0.5 and 1.5 as unaffected VOCs, and three peaks with a ratio <0.5 as resorbed VOCs. The peak intensity of 12 expired VOCs changed significantly during the 24 h measurement. These results represent the basis for future intervention studies. Notably, online VOC analysis with MCC-IMS is possible over 24 h in ventilated rats and allows different experimental approaches"
Keywords:		"Animals Exhalation/physiology Male Models, Animal Rats Rats, Sprague-Dawley *Respiration, Artificial Spectrum Analysis/methods Volatile Organic Compounds/*metabolism;"
Notes:		"MedlineAlbrecht, F W Huppe, T Fink, T Maurer, F Wolf, A Wolf, B Volk, T Baumbach, J I Kreuer, S eng Research Support, Non-U.S. Gov't England 2015/03/10 J Breath Res. 2015 Mar 9; 9(1):016007. doi: 10.1088/1752-7155/9/1/016007"