| Title: | Sex- and Tissue-Specific Expression Profiles of Odorant Binding Protein and Chemosensory Protein Genes in Bradysia odoriphaga (Diptera: Sciaridae) |
| Author(s): | Zhao Y; Ding J; Zhang Z; Liu F; Zhou C; Mu W; |
| Address: | "College of Plant Protection, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, Shandong Agricultural University, Tai'an, China. College of Horticultural Science and Engineering, Shandong Agricultural University, Tai'an, China. College of Plant Protection, Shandong Agricultural University, Tai'an, China" |
| ISSN/ISBN: | 1664-042X (Print) 1664-042X (Electronic) 1664-042X (Linking) |
| Abstract: | "Bradysia odoriphaga is an agricultural pest insect affecting the production of Chinese chive and other liliaceous vegetables in China, and it is significantly attracted by sex pheromones and the volatiles derived from host plants. Despite verification of this chemosensory behavior, however, it is still unknown how B. odoriphaga recognizes these volatile compounds on the molecular level. Many of odorant binding proteins (OBPs) and chemosensory proteins (CSPs) play crucial roles in olfactory perception. Here, we identified 49 OBP and 5 CSP genes from the antennae and body transcriptomes of female and male adults of B. odoriphaga, respectively. Sequence alignment and phylogenetic analysis among Dipteran OBPs and CSPs were analyzed. The sex- and tissue-specific expression profiles of 54 putative chemosensory genes among different tissues were investigated by quantitative real-time PCR (qRT-PCR). qRT-PCR analysis results suggested that 22 OBP and 3 CSP genes were enriched in the antennae, indicating they might be essential for detection of general odorants and pheromones. Among these antennae-enriched genes, nine OBPs (BodoOBP2/4/6/8/12/13/20/28/33) were enriched in the male antennae and may play crucial roles in the detection of sex pheromones. Moreover, some OBP and CSP genes were enriched in non-antennae tissues, such as in the legs (BodoOBP3/9/19/21/34/35/38/39/45 and BodoCSP1), wings (BodoOBP17/30/32/37/44), abdomens and thoraxes (BodoOBP29/36), and heads (BodoOBP14/23/31 and BodoCSP2), suggesting that these genes might be involved in olfactory, gustatory, or other physiological processes. Our findings provide a starting point to facilitate functional research of these chemosensory genes in B. odoriphaga at the molecular level" |
| Keywords: | Bradysia odoriphaga chemosensory protein expression profiles analysis odorant binding protein transcriptomes; |
| Notes: | "PubMed-not-MEDLINEZhao, Yunhe Ding, Jinfeng Zhang, Zhengqun Liu, Feng Zhou, Chenggang Mu, Wei eng Switzerland 2018/04/19 Front Physiol. 2018 Apr 3; 9:107. doi: 10.3389/fphys.2018.00107. eCollection 2018" |
