| Title: | Simultaneous determination of five mercapturic acid derived from volatile organic compounds in human urine by LC-MS/MS and its application to relationship study |
| Author(s): | Zhang X; Xiong W; Shi L; Hou H; Hu Q; |
| Address: | "China National Tobacco Quality Supervision & Test Center, No. 2 Fengyang Street, Zhengzhou High & New Technology Industries Development Zone, 450001 Zhengzhou, People's Republic of China. China National Tobacco Quality Supervision & Test Center, No. 2 Fengyang Street, Zhengzhou High & New Technology Industries Development Zone, 450001 Zhengzhou, People's Republic of China; Department of Clinical Pharmacy, Pharmacy School of Sichuan University, Chengdu, People's Republic of China. China National Tobacco Quality Supervision & Test Center, No. 2 Fengyang Street, Zhengzhou High & New Technology Industries Development Zone, 450001 Zhengzhou, People's Republic of China. Electronic address: qsfctc@163.com. China National Tobacco Quality Supervision & Test Center, No. 2 Fengyang Street, Zhengzhou High & New Technology Industries Development Zone, 450001 Zhengzhou, People's Republic of China. Electronic address: huqy1965@163.com" |
| Journal Title: | J Chromatogr B Analyt Technol Biomed Life Sci |
| DOI: | 10.1016/j.jchromb.2014.07.013 |
| ISSN/ISBN: | 1873-376X (Electronic) 1570-0232 (Linking) |
| Abstract: | "Acrylonitrile, acrolein, 1,3-butadiene, benzene, and crotonaldehyde are hazard volatile organic compounds in tobacco smoke, which can be metabolized to mercapturic acids (MAs) excreted in urine. MAs are can be regarded as important and specific biomarkers to evaluate exposure to those carcinogenic volatile organic compounds. A simultaneous determination of N-acetyl-S-2-cyanoethyl-cysteine (CEMA), 3-hydroxypropyl)-L-cysteine (3-HPMA), N-acetyl-S-(3,4-dihydroxybutyl)-L-cysteine (DHBMA), N-acetyl-S-(phenyl)-L-cysteine (SPMA) and 3-hydroxy-1-methylpropylmercapturic acid (HMPMA) derived from five volatile organic compounds by column-switching LC-MS/MS has been described. MAs were concentrated and cleaned up by an online reusable pre-column packed with restricted access material. The intra- and inter-day precisions of the method ranged from 0.7% to 15.2%. The LODs was 0.013-0.053 ng/mL. The recovery of the whole analytical procedure ranged from 79.3% to 116%. After validation, this method was successfully applied to urine samples from smokers (n=246) and nonsmokers (n=58). The results showed MAs in urine from smokers were significantly higher than that in nonsmoker except for SPMA. Urinary CEMA significantly correlated with 3-HPMA (r=0.763, P<0.0001) and HMPMA (r=0.910, P<0.0001). CEMA, 3-HPMA and HMPMA are potential biomarkers to distinguish the differences between smokers and nonsmokers" |
| Keywords: | "Acetylcysteine/*analogs & derivatives/*urine Biomarkers/*urine Chromatography, Liquid/*methods Humans Limit of Detection Linear Models Reproducibility of Results Smoking/urine Tandem Mass Spectrometry/*methods Column-switching Lc-ms-ms Mercapturic acids U;" |
| Notes: | "MedlineZhang, Xiaotao Xiong, Wei Shi, Longkai Hou, Hongwei Hu, Qingyuan eng Research Support, Non-U.S. Gov't Netherlands 2014/08/05 J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Sep 15; 967:102-9. doi: 10.1016/j.jchromb.2014.07.013. Epub 2014 Jul 18" |
