Title: | Rapid characterization of the volatile profiles in Pu-erh tea by gas phase electronic nose and microchamber/thermal extractor combined with TD-GC-MS |
Author(s): | Yang Y; Rong Y; Liu F; Jiang Y; Deng Y; Dong C; Yuan H; |
Address: | "Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China. Yunnan Shuangjiang Mengku Tea Co., Ltd., Lincang, China" |
ISSN/ISBN: | 1750-3841 (Electronic) 0022-1147 (Linking) |
Abstract: | "Aroma plays an important role in the quality of Pu-erh tea. However, the quality evaluation of Pu-erh tea aroma is heavily relied on the experience of sensory evaluation, and the theoretical research is relatively scarce. In the present work, the volatile compounds in Pu-erh tea were characterized by using gas phase electronic nose (e-nose) and microchamber/thermal extractor (micro-CTE) combined with thermal desorption coupled to gas chromatography-mass spectrometry (TD-GC-MS). A satisfactory discrimination model (R(2) Y = 0.95, Q(2) = 0.807) was obtained by using orthogonal partial least squares discriminant analysis (OPLS-DA) based on the odor fingerprint of different brands of Pu-erh tea. In addition, based on the double criterion of multivariate analysis with VIP >1.0 and univariate analysis with p = 0.001, 39 volatile components were identified to contribute greatly to the discrimination of five brands of Pu-erh tea. The results suggested that gas phase e-nose and micro-CTE combined with TD-GC/MS were simple, rapid techniques to characterize the volatile compounds in Pu-erh tea and were allowed to effectively distinguish different brands of Pu-erh tea, which would provide an important reference on the quality assessment of Pu-erh tea. PRACTICAL APPLICATION: This work demonstrates that the volatile compounds in Pu-erh tea are simply and rapidly characterized by using micro-CTE/TD-GC/MS and gas phase e-nose, allowing to effectively distinguish different brands of Pu-erh tea, which can provide an important reference for the quality assessment and authentication of Pu-erh tea" |
Keywords: | Discriminant Analysis *Electronic Nose Gas Chromatography-Mass Spectrometry/*methods Multivariate Analysis Odorants/*analysis Tea/*chemistry Volatile Organic Compounds/*analysis Pu-erh tea electronic nose gas chromatography-mass spectrometry microchamber/; |
Notes: | "MedlineYang, Yanqin Rong, Yuting Liu, Fuqiao Jiang, Yongwen Deng, Yuliang Dong, Chunwang Yuan, Haibo eng 31902083/National Natural Science Foundation of China/ 31972465/National Natural Science Foundation of China/ CAAS-ASTIP-TRICAAS/the Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences/ LQ18C160006/Natural Science Foundation of Zhejiang Province/ 2021/05/01 J Food Sci. 2021 Jun; 86(6):2358-2373. doi: 10.1111/1750-3841.15723. Epub 2021 Apr 30" |