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Theriogenology

Title:		Oxidative stress mediates heat-induced changes of tight junction proteins in porcine sertoli cells via inhibiting CaMKKbeta-AMPK pathway
Author(s):		Yang WR; Li BB; Hu Y; Zhang L; Wang XZ;
Address:		"Chongqing Key Laboratory of Forage & Herbivore, College of Animal Science and Technology, Southwest University, Chongqing, 400716, PR China; Institute of Ecological Research, Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong, Sichuan, 637002, PR China. Geomathematics Key Laboratory of Sichuan Province, Chengdu University of Technology, Chengdu, 610059, PR China. Chongqing Key Laboratory of Forage & Herbivore, College of Animal Science and Technology, Southwest University, Chongqing, 400716, PR China. Institute of Ecological Research, Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong, Sichuan, 637002, PR China. Chongqing Key Laboratory of Forage & Herbivore, College of Animal Science and Technology, Southwest University, Chongqing, 400716, PR China. Electronic address: xianzhong_wang@aliyun.com"
Journal Title:		Theriogenology
Year:		2020
Volume:		20190919
Issue:
Page Number:		104 - 113
DOI:		10.1016/j.theriogenology.2019.09.031
ISSN/ISBN:		1879-3231 (Electronic) 0093-691X (Linking)
Abstract:		"Heat stress causes reversible changes in tight junction proteins in immature Sertoli cells via inhibition of the AMPK signaling pathway; these effects are accompanied by an increase in the early apoptotic rate and decrease in the cell viability of Sertoli cells. Since heat stress is known to also cause oxidative damage, in the present study, we investigated whether the earlier mentioned effects of heat stress were brought about via the induction of oxidative stress in boar Sertoli cells. Immature Sertoli cells obtained from 3-week-old piglets were subjected to heat treatment (43?ª+ degrees C, 30?ª+min), and the percentage of ROS-positive cells, the malonaldehyde (MDA) concentration, and the activity of the antioxidases, including superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) were measured. Next, the Sertoli cells were treated with N-acetyl-l-cysteine (NAC) (1?ª+mmol/L, 2?ª+h), an antioxidant agent, before they were exposed to heat stress. The effects of NAC on ROS accumulation, MDA levels, antioxidase activity, the CaMKKbeta-AMPK signaling pathway and expression of tight junction proteins were assessed. The results showed that heat stress reversibly increased the percentage of ROS-positive cells and MDA levels, and decreased the activity of SOD, GSH-Px, and CAT. Pretreatment with NAC abrogated these effects of heat stress. Additionally, NAC reversed the heat stress-induced decrease in the expression of CaMKKbeta and dephosphorylation of AMPK. NAC also obviously rescued the heat stress-induced downregulation of tight junction proteins (claudin-11, JAM-A, occludin, and ZO-1) both at the mRNA and protein level. In conclusion, the findings indicate that oxidative damage participates in heat stress-induced downregulation of tight junction proteins in Sertoli cells by inhibiting the CaMKKbeta-AMPK axis. Further, NAC reversed the effects of heat stress on tight junction proteins; this means that it has potential as a protective agent that can prevent reproductive dysfunction in boars under conditions of heat stress"
Keywords:		AMP-Activated Protein Kinases/genetics/metabolism Animals Calcium-Calmodulin-Dependent Protein Kinase Kinase/genetics/*metabolism Cell Survival Glutathione Peroxidase/metabolism *Hot Temperature Male Malondialdehyde *Oxidative Stress Phosphorylation React;
Notes:		"MedlineYang, Wei-Rong Li, Bin-Bin Hu, Yu Zhang, Long Wang, Xian-Zhong eng 2019/10/07 Theriogenology. 2020 Jan 15; 142:104-113. doi: 10.1016/j.theriogenology.2019.09.031. Epub 2019 Sep 19"