| Title: | Regeneration of duckweed (Lemna turonifera) involves genetic molecular regulation and cyclohexane release |
| Author(s): | Yang L; Sun J; Yan C; Wu J; Wang Y; Ren Q; Wang S; Ma X; Zhao L; Sun J; |
| Address: | "Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin, China. Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, Tianjin, China" |
| DOI: | 10.1371/journal.pone.0254265 |
| ISSN/ISBN: | 1932-6203 (Electronic) 1932-6203 (Linking) |
| Abstract: | "Plant regeneration is important for vegetative propagation, detoxification and the obtain of transgenic plant. We found that duckweed regeneration could be enhanced by regenerating callus. However, very little is known about the molecular mechanism and the release of volatile organic compounds (VOCs). To gain a global view of genes differently expression profiles in callus and regenerating callus, genetic transcript regulation has been studied. Auxin related genes have been significantly down-regulated in regenerating callus. Cytokinin signal pathway genes have been up-regulated in regenerating callus. This result suggests the modify of auxin and cytokinin balance determines the regenerating callus. Volatile organic compounds release has been analysised by gas chromatography/ mass spectrum during the stage of plant regeneration, and 11 kinds of unique volatile organic compounds in the regenerating callus were increased. Cyclohexane treatment enhanced duckweed regeneration by initiating root. Moreover, Auxin signal pathway genes were down-regulated in callus treated by cyclohexane. All together, these results indicated that cyclohexane released by regenerating callus promoted duckweed regeneration. Our results provide novel mechanistic insights into how regenerating callus promotes regeneration" |
| Keywords: | "Araceae/chemistry/*growth & development Cyclohexanes/*metabolism Cytokinins/metabolism *Gene Expression Regulation, Plant Indoleacetic Acids/metabolism Plant Growth Regulators/*metabolism Plant Proteins/genetics/*metabolism *Regeneration Signal Transducti;" |
| Notes: | "MedlineYang, Lin Sun, Jinge Yan, Congyu Wu, Junyi Wang, Yaya Ren, Qiuting Wang, Shen Ma, Xu Zhao, Ling Sun, Jinsheng eng 2022/01/07 PLoS One. 2022 Jan 6; 17(1):e0254265. doi: 10.1371/journal.pone.0254265. eCollection 2022" |
