| Title: | The regulator of G-protein signalling Thn1 links pheromone response to volatile production in Schizophyllum commune |
| Author(s): | Wirth S; Kunert M; Ahrens LM; Krause K; Broska S; Paetz C; Kniemeyer O; Jung EM; Boland W; Kothe E; |
| Address: | "Friedrich Schiller University Jena, Institute of Microbiology, Microbial Communication, Neugasse 25, 07743, Jena, Germany. Max Planck Institute for Chemical Ecology, Bioorganic Chemistry, Hans-Knoll-Strasse 8, 07745, Jena, Germany. Leibnitz Institute for Natural Product Research and Infection Biology - Hans Knoll Institute, Molecular and Applied Microbiology, Adolf-Reichwein-Strasse 23, 07745, Jena, Germany" |
| ISSN/ISBN: | 1462-2920 (Electronic) 1462-2912 (Linking) |
| Abstract: | "The regulator of G-protein signalling, Thn1, is involved in sexual development through pheromone signalling in the mushroom forming basidiomycete Schizophyllum commune affecting hyphal morphology and mating interactions. Thn1 plays a key role in coordinating sesquiterpene production, pheromone response and sexual development. The gene thn1 is transcriptionally regulated in response to mating with a role in clamp cell development and hydrophobin gene transcription. Further, it negatively regulates cAMP signalling and secondary metabolism. Disruption of thn1 affects dikaryotization by reducing clamp fusion and development with predominant non-fused pseudoclamps. Enhanced protein kinase A (PKA) activities in Deltathn1 strains indicate that Thn1 regulates pheromone signalling by de-activating G-protein alpha subunits, which control cAMP-dependent PKA. The repressed formation of aerial hyphae could be linked to a reduced metabolic activity and to a transcriptional down-regulation of hyd6 and sc3 hydrophobin genes. Thn1 was also shown to be necessary for the biosynthesis of sesquiterpenes and an altered spectrum of sesquiterpenes in Deltathn1 is linked to transcriptional up-regulation of biosynthesis genes. Proteome analysis indicated changes in cytoskeletal structure affecting actin localization, linking the major regulator Thn1 to growth and development of S. commune. The results support a role for Thn1 in G-protein signalling connecting development and secondary metabolism" |
| Keywords: | "Cyclic AMP/metabolism Fungal Proteins/genetics/*metabolism GTP-Binding Proteins/*genetics/metabolism Gene Expression Regulation, Fungal Hyphae/genetics/growth & development/metabolism Pheromones/*metabolism Schizophyllum/genetics/growth & development/*met;" |
| Notes: | "MedlineWirth, Sophia Kunert, Maritta Ahrens, Lisa-Marija Krause, Katrin Broska, Selina Paetz, Christian Kniemeyer, Olaf Jung, Elke-Martina Boland, Wilhelm Kothe, Erika eng Jena School for Microbial Communication (JSMC)/International Friedrich Schiller University Jena/International Research Support, Non-U.S. Gov't England 2018/08/01 Environ Microbiol. 2018 Oct; 20(10):3684-3699. doi: 10.1111/1462-2920.14369. Epub 2018 Sep 17" |
