| Title: | Pheromone-regulated sumoylation of transcription factors that mediate the invasive to mating developmental switch in yeast |
| Address: | "Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599-7260, USA" |
| ISSN/ISBN: | 0021-9258 (Print) 0021-9258 (Linking) |
| Abstract: | "A fundamental question in biology is how different signaling pathways use common signaling proteins to attain different developmental outcomes. The yeast transcription factor Ste12 is required in at least two distinct signaling processes, each regulated by many of the same protein kinases. Whereas Ste12-Ste12 homodimers promote transcription of genes required for mating, Ste12-Tec1 heterodimers activate genes required for invasive growth. We report that Ste12 and Tec1 undergo covalent modification by the ubiquitin-related modifier SUMO. Stimulation by mating pheromone promotes sumoylation of Ste12 and diminishes the sumoylation of Tec1. In the absence of sumoylation Tec1 is more rapidly degraded. We propose that pheromone-regulated sumoylation of Ste12 and Tec1 promotes a developmental switch from the invasive to the mating differentiation program" |
| Keywords: | "Biological Assay Cell Differentiation Cell Proliferation DNA/metabolism DNA-Binding Proteins/*physiology Dimerization Gene Expression Regulation, Developmental *Gene Expression Regulation, Fungal *Genes, Mating Type, Fungal Immunoprecipitation MAP Kinase;" |
| Notes: | "MedlineWang, Yuqi Dohlman, Henrik G eng R01 GM055316/GM/NIGMS NIH HHS/ R01 GM059167/GM/NIGMS NIH HHS/ GM 055316/GM/NIGMS NIH HHS/ GM 059167/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't 2005/11/25 J Biol Chem. 2006 Jan 27; 281(4):1964-9. doi: 10.1074/jbc.M508985200. Epub 2005 Nov 23" |
