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J Insect Sci

Title:		Specificity of the receptor for the major sex pheromone component in Heliothis virescens
Author(s):		Vasquez GM; Syed Z; Estes PA; Leal WS; Gould F;
Address:		"1 Department of Entomology and W. M. Keck Center for Behavioral Biology, North Carolina State University, Raleigh, NC 27695, USA"
Journal Title:		J Insect Sci
Year:		2013
Volume:		13
Issue:
Page Number:		160 -
DOI:		10.1673/031.013.16001
ISSN/ISBN:		1536-2442 (Electronic) 1536-2442 (Linking)
Abstract:		"In a previous study, the Drosophila melanogaster OR67d(GAL4);UAS system was used to functionally characterize the receptor for the major component of the sex pheromone in the tobacco budworm, Heliothis virescens Fabricius (Lepidoptera: Noctuidae), HvOR13. Electrophysiological and behavioral assays showed that transgenic flies expressing HvOR13 responded to (Z)-11-hexadecenal (Z11-16:Ald). However, tests were not performed to determine whether these flies would also respond to secondary components of the H. virescens sex pheromone. Thus, in this study the response spectrum of HvOR13 expressed in this system was examined by performing single cell recordings from odor receptor neuron in trichoid T1 sensilla on antennae of two Or67d(GAL4 [1]); UAS-HvOR13 lines stimulated with Z11-16:Ald and six H. virescens secondary pheromone components. Fly courtship assays were also performed to examine the behavioral response of the Or67d(GAL4[1]); UAS-HvOR13 flies to Z11-16:Ald and the secondary component Z9-14:Ald. Our combined electrophysiological and behavioral studies indicated high specificity and sensitivity of HvOR13 to Z11-16:Ald. Interestingly, a mutation leading to truncation in the HvOR13 C-terminal region affected but did not abolish pheromone receptor response to Z11-16:Ald. The findings are assessed in relationship to other HvOR13 heterologous expression studies, and the role of the C-terminal domain in receptor function is discussed. A third line expressing HvOR15 was also tested but did not respond to any of the seven pheromone components"
Keywords:		"Animals Animals, Genetically Modified/genetics/physiology Arthropod Antennae/*physiology Drosophila melanogaster/genetics/physiology Gene Expression Insect Proteins/*genetics/metabolism Molecular Sequence Data Moths/genetics/*physiology Receptors, Odorant;"
Notes:		"MedlineVasquez, Gissella M Syed, Zainulabeuddin Estes, Patricia A Leal, Walter S Gould, Fred eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2014/04/30 J Insect Sci. 2013; 13:160. doi: 10.1673/031.013.16001"