| Title: | Synthetic pheromone biosynthesis activating neuropeptide gene expressed in a baculovirus expression system |
| Author(s): | Vakharia VN; Raina AK; Kingan TG; Kempe TG; |
| Address: | "Center for Agricultural Biotechnology of Maryland Biotechnology Institute and College of Veterinary Medicine, University of Maryland, College Park 20742, USA" |
| DOI: | 10.1016/0965-1748(94)00097-2 |
| ISSN/ISBN: | 0965-1748 (Print) 0965-1748 (Linking) |
| Abstract: | "A synthetic gene of the pheromone biosynthesis activating neuropeptide (PBAN) of corn earworm Helicoverpa zea, with and without a signal sequence of the cuticle protein of Drosophila melanogaster, was cloned behind the polyhedrin promoter of AcMNPV. Two recombinant baculoviruses were constructed and used to infect a number of insect cell lines including Sf9 and 5B1-4. High pheromonotropic activity was consistently obtained from 5B1-4 cell culture that was infected with the recombinant baculovirus vINV-4 containing the signal sequence. The PBAN gene-product was isolated by HPLC and analyzed by electrospray ionization mass spectrometry. Low levels of biological activity obtained from Sf9 cells infected with the recombinant virus vPBAN may be due to lack of proper amidation at the C-terminus of the expressed peptide or rapid proteolytic degradation of the product" |
| Keywords: | Amino Acid Sequence Animals Base Sequence Cell Line DNA Drosophila melanogaster/genetics Molecular Sequence Data Neuropeptides/*genetics/metabolism Nucleopolyhedroviruses/*genetics Protein Sorting Signals/genetics Recombinant Fusion Proteins/genetics Sex; |
| Notes: | "MedlineVakharia, V N Raina, A K Kingan, T G Kempe, T G eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 1995/05/01 Insect Biochem Mol Biol. 1995 May; 25(5):583-9. doi: 10.1016/0965-1748(94)00097-2" |
